PUBLICATION
Intestinal growth and differentiation in zebrafish
- Authors
- Wallace, K.N., Akhter, S., Smith, E.M., Lorent, K., and Pack, M.
- ID
- ZDB-PUB-050120-6
- Date
- 2005
- Source
- Mechanisms of Development 122(2): 157-73 (Journal)
- Registered Authors
- Lorent, Kristin, Pack, Michael, Wallace, Kenneth
- Keywords
- Zebrafish; Intestine; Development; Differentiation
- MeSH Terms
-
- Male
- Cell Proliferation
- Models, Biological
- Epithelial Cells/cytology
- Muscle, Smooth/cytology
- Muscle, Smooth/metabolism
- Enteric Nervous System/embryology
- Enteric Nervous System/growth & development
- Animals
- Female
- Immunohistochemistry
- Antimetabolites/pharmacology
- Intestines/embryology*
- Intestines/growth & development*
- Intestines/metabolism
- Mutation
- Phenotype
- Epithelium/embryology
- Epithelium/growth & development
- RNA/metabolism
- Horseradish Peroxidase/pharmacology
- Body Patterning
- In Situ Hybridization
- Bromodeoxyuridine/pharmacology
- Cell Differentiation
- Neurons/metabolism
- Zebrafish
- Time Factors
- Gene Expression Regulation, Developmental*
- PubMed
- 15652704 Full text @ Mech. Dev.
Citation
Wallace, K.N., Akhter, S., Smith, E.M., Lorent, K., and Pack, M. (2005) Intestinal growth and differentiation in zebrafish. Mechanisms of Development. 122(2):157-73.
Abstract
Intestinal development in amniotes is driven by interactions between progenitor cells derived from the three primary germ layers. Genetic analyses and gene targeting experiments in zebrafish offer a novel approach to dissect such interactions at a molecular level. Here we show that intestinal anatomy and architecture in zebrafish closely resembles the anatomy and architecture of the mammalian small intestine. The zebrafish intestine is regionalized and the various segments can be identified by epithelial markers whose expression is already segregated at the onset of intestinal differentiation. Differentiation of cells derived from the three primary germ layers begins more or less contemporaneously, and is preceded by a stage in which there is rapid cell proliferation and maturation of epithelial cell polarization. Analysis of zebrafish mutants with altered epithelial survival reveals that seemingly related single gene defects have different effects on epithelial differentiation and smooth muscle and enteric nervous system development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping