PUBLICATION
Hedgehog Signaling Is Required for Adult Blood Stem Cell Formation in Zebrafish Embryos
- Authors
- Gering, M., and Patient, R.
- ID
- ZDB-PUB-050303-2
- Date
- 2005
- Source
- Developmental Cell 8(3): 389-400 (Journal)
- Registered Authors
- Gering, Martin, Patient, Roger K.
- Keywords
- none
- MeSH Terms
-
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/physiology
- Proto-Oncogene Proteins/metabolism
- Vascular Endothelial Growth Factor A/metabolism
- Animals
- Hematopoietic Stem Cells/cytology*
- Hematopoietic Stem Cells/metabolism
- Neovascularization, Physiologic/drug effects
- Neovascularization, Physiologic/physiology
- Cell Movement/drug effects
- Cell Movement/physiology
- Zebrafish
- T-Lymphocytes/cytology*
- T-Lymphocytes/metabolism
- Mutation/genetics
- DNA-Binding Proteins/metabolism
- Receptors, Notch
- Hematopoiesis/drug effects
- Hematopoiesis/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Membrane Proteins/metabolism
- Signal Transduction/drug effects
- Signal Transduction/physiology*
- Hedgehog Proteins
- Veratrum Alkaloids/toxicity
- Core Binding Factor Alpha 2 Subunit
- Body Patterning/drug effects
- Body Patterning/physiology
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Transcription Factors/metabolism
- PubMed
- 15737934 Full text @ Dev. Cell
Citation
Gering, M., and Patient, R. (2005) Hedgehog Signaling Is Required for Adult Blood Stem Cell Formation in Zebrafish Embryos. Developmental Cell. 8(3):389-400.
Abstract
Studies with embryonic explants and embryonic stem cells have suggested a role for Hedgehog (Hh) signaling in hematopoiesis. However, targeted deletion of Hh pathway components in the mouse has so far failed to provide in vivo evidence. Here we show that zebrafish embryos mutant in the Hh pathway or treated with the Hh signaling inhibitor cyclopamine display defects in adult hematopoietic stem cell (HSC) formation but not in primitive hematopoiesis. Hh is required in the trunk at three consecutive stages during vascular development: for the medial migration of endothelial progenitors of the dorsal aorta (DA), for arterial gene expression, and for the formation of intersomitic vessel sprouts. Interference with Hh signaling during the first two stages also interferes with HSC formation. Furthermore, HSC and DA formation also share Vegf and Notch requirements, which further distinguishes them from primitive hematopoiesis and underlines their close relationship during vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping