PUBLICATION
Histone deacetylase 1 regulates retinal neurogenesis in zebrafish by suppressing Wnt and Notch signaling pathways
- Authors
- Yamaguchi, M., Tonou-Fujimori, N., Komori, A., Maeda, R., Nojima, Y., Li, H., Okamoto, H., and Masai, I.
- ID
- ZDB-PUB-050610-14
- Date
- 2005
- Source
- Development (Cambridge, England) 32(13): 3027-3043 (Journal)
- Registered Authors
- Komori, Atsuko, Masai, Ichiro, Okamoto, Hitoshi, Yamaguchi, Masahiro
- Keywords
- Cell cycle, Danio rerio, Histone deacetylase, Neurogenesis, Notch, Retina, Wnt, Zebrafish
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/genetics
- Cell Differentiation/physiology
- Cell Cycle/genetics
- Molecular Sequence Data
- Intercellular Signaling Peptides and Proteins/metabolism*
- Amino Acid Sequence
- Cyclin-Dependent Kinase Inhibitor p27
- Histone Deacetylase 1
- Growth Inhibitors/genetics
- Growth Inhibitors/physiology
- Histone Deacetylases/genetics
- Histone Deacetylases/metabolism*
- Cell Cycle Proteins/metabolism
- Down-Regulation/genetics
- Down-Regulation/physiology*
- Cyclin D1/metabolism
- Signal Transduction/genetics*
- Tumor Suppressor Proteins/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Retina/cytology
- Retina/embryology*
- Mutation
- Receptors, Notch
- Wnt Proteins
- Cell Proliferation
- Animals
- Membrane Proteins/antagonists & inhibitors
- Membrane Proteins/metabolism*
- PubMed
- 15944187 Full text @ Development
Citation
Yamaguchi, M., Tonou-Fujimori, N., Komori, A., Maeda, R., Nojima, Y., Li, H., Okamoto, H., and Masai, I. (2005) Histone deacetylase 1 regulates retinal neurogenesis in zebrafish by suppressing Wnt and Notch signaling pathways. Development (Cambridge, England). 32(13):3027-3043.
Abstract
In the developing vertebrate retina, progenitor cells initially proliferate but begin to produce postmitotic neurons when neuronal differentiation occurs. However, the mechanism that determines whether retinal progenitor cells continue to proliferate or exit from the cell cycle and differentiate is largely unknown. Here, we report that histone deacetylase 1 (Hdac1) is required for the switch from proliferation to differentiation in the zebrafish retina. We isolated a zebrafish mutant, ascending and descending (add), in which retinal cells fail to differentiate into neurons and glial cells but instead continue to proliferate. The cloning of the add gene revealed that it encodes Hdac1. Furthermore, the ratio of the number of differentiating cells to that of proliferating cells increases in proportion to Hdac activity, suggesting that Hdac proteins regulate a crucial step of retinal neurogenesis in zebrafish. Canonical Wnt signaling promotes the proliferation of retinal cells in zebrafish, and Notch signaling inhibits neuronal differentiation through the activation of a neurogenic inhibitor, Hairy/Enhancer-of-split (Hes). We found that both the Wnt and Notch/Hes pathways are activated in the add mutant retina. The cell-cycle progression and the upregulation of Hes expression in the add mutant retina can be inhibited by the blockade of Wnt and Notch signaling, respectively. These data suggest that Hdac1 antagonizes these pathways to promote cell-cycle exit and the subsequent neurogenesis in zebrafish retina. Taken together, these data suggest that Hdac1 functions as a dual switch that suppresses both cell-cycle progression and inhibition of neurogenesis in the zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping