PUBLICATION
Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain
- Authors
- Berndt, J.D., and Halloran, M.C.
- ID
- ZDB-PUB-060921-15
- Date
- 2006
- Source
- Development (Cambridge, England) 133(20): 3983-3992 (Journal)
- Registered Authors
- Berndt, Jason, Halloran, Mary
- Keywords
- Neural crest, Zebrafish, Semaphorin, TCF, Cell cycle, Cyclin
- MeSH Terms
-
- Rhombencephalon/chemistry
- Rhombencephalon/cytology
- Rhombencephalon/growth & development*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Mutation
- Cyclins/genetics
- Neural Crest/chemistry
- Neural Crest/cytology
- Neural Crest/growth & development*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Gene Expression
- Wnt Proteins/metabolism
- Cell Movement/genetics
- Semaphorins/antagonists & inhibitors
- Semaphorins/genetics
- Semaphorins/physiology*
- Cell Proliferation
- Animals
- Nerve Growth Factors/antagonists & inhibitors
- Nerve Growth Factors/genetics
- Nerve Growth Factors/physiology*
- Zebrafish/growth & development*
- Pluripotent Stem Cells/chemistry
- Pluripotent Stem Cells/cytology
- Pluripotent Stem Cells/physiology*
- Body Patterning
- PubMed
- 16971468 Full text @ Development
Citation
Berndt, J.D., and Halloran, M.C. (2006) Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain. Development (Cambridge, England). 133(20):3983-3992.
Abstract
Neural crest cells (NCCs) are pluripotent migratory cells that are crucial to the development of the peripheral nervous system, pigment cells and craniofacial cartilage and bone. NCCs are specified within the dorsal ectoderm and undergo an epithelial to mesenchymal transition (EMT) in order to migrate to target destinations where they differentiate. Here we report a role for a member of the semaphorin family of cell guidance molecules in NCC development. Morpholino-mediated knockdown of Sema3d inhibits the proliferation of hindbrain neuroepithelial cells. In addition, Sema3d knockdown reduces markers of migratory NCCs and disrupts NCC-derived tissues. Similarly, expression of a dominant-repressor form of TCF (DeltaTCF) reduces hindbrain cell proliferation and leads to a disruption of migratory NCC markers. Moreover, expression of DeltaTCF downregulates sema3d RNA expression. Finally, Sema3d overexpression rescues reduced proliferation caused by DeltaTCF expression, suggesting that Sema3d lies downstream of Wnt/TCF signaling in the molecular pathway thought to control cell cycle in NCC precursors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping