PUBLICATION
Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: III. 2-O-sulfotransferase and C5-epimerases
- Authors
- Cadwallader, A.B., and Yost, H.J.
- ID
- ZDB-PUB-070122-2
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(2): 581-6 (Journal)
- Registered Authors
- Cadwallader, Adam, Yost, H. Joseph
- Keywords
- zebrafish, heparan sulfate, 2-O-sulfotransferase, proteoglycan, 2-OST, C5-epimerase, Glce, GlceA, GlceB
- MeSH Terms
-
- Racemases and Epimerases/metabolism*
- Phylogeny*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Sulfotransferases/genetics
- Sulfotransferases/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Cluster Analysis
- Gene Expression Regulation, Developmental*
- Sequence Analysis, DNA
- Base Sequence
- Animals
- Molecular Sequence Data
- Amino Acid Sequence
- In Situ Hybridization
- Species Specificity
- Cloning, Molecular
- PubMed
- 17195182 Full text @ Dev. Dyn.
Citation
Cadwallader, A.B., and Yost, H.J. (2007) Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: III. 2-O-sulfotransferase and C5-epimerases. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(2):581-6.
Abstract
Heparan sulfate (HS) is an unbranched chain of repetitive disaccharides, which specifically binds ligands when attached to the cell surface or secreted extracellularly. HS chains contain sulfated domains, termed the HS fine structure, which give HS specific binding affinities for extracellular ligands. HS 2-O-sulfotransferase (2-OST) catalyzes the transfer of sulfate groups to the 2-O position of uronic acid residues of HS. We report here the characterization and developmental expression patterns of 2-OST in several tissues/organs throughout early zebrafish development, including early cleavage stages, eyes, somites, brain, internal organ primordial, and pectoral fin. The 2-OST gene has spatially and temporally distinct expression, which is a surprise given the essential role of 2-OST in HS fine structure formation. Furthermore, although 2-OST and C5-epimerase are predicted to be interdependent for protein translocation from the endoplasmic reticulum to the Golgi, their expression is not coordinately regulated during zebrafish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping