PUBLICATION
Ischemia Is Not Required for Arteriogenesis in Zebrafish Embryos
- Authors
- Gray, C., Packham, I.M., Wurmser, F., Eastley, N.C., Hellewell, P.G., Ingham, P.W., Crossman, D.C., and Chico, T.J.
- ID
- ZDB-PUB-070806-19
- Date
- 2007
- Source
- Arterioscler. Thromb. Vasc. Biol. 27(10): 2135-2141 (Journal)
- Registered Authors
- Chico, Tim J., Gray, Caroline, Ingham, Philip
- Keywords
- collateral circulation, angiogenesis, nitric oxide, blood flow, zebrafish
- MeSH Terms
-
- Aortic Diseases/embryology
- Aortic Diseases/genetics
- Aortic Diseases/metabolism
- Aortic Diseases/physiopathology*
- Neovascularization, Physiologic*
- Nitric Oxide/metabolism
- Nitric Oxide Synthase/metabolism
- Mutation
- Time Factors
- Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
- Ischemia/embryology
- Ischemia/genetics
- Ischemia/metabolism
- Ischemia/physiopathology*
- RNA, Messenger/metabolism
- Arteries/embryology
- Arteries/growth & development*
- Arteries/metabolism
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Myeloid Cells/metabolism
- Disease Models, Animal
- Cell Hypoxia
- Microscopy, Confocal
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Arterial Occlusive Diseases/embryology
- Arterial Occlusive Diseases/genetics
- Arterial Occlusive Diseases/metabolism
- Arterial Occlusive Diseases/physiopathology*
- Collateral Circulation*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Proto-Oncogene Protein c-fli-1/genetics
- Proto-Oncogene Protein c-fli-1/metabolism
- Animals, Genetically Modified
- PubMed
- 17656667 Full text @ Arterioscler. Thromb. Vasc. Biol.
Citation
Gray, C., Packham, I.M., Wurmser, F., Eastley, N.C., Hellewell, P.G., Ingham, P.W., Crossman, D.C., and Chico, T.J. (2007) Ischemia Is Not Required for Arteriogenesis in Zebrafish Embryos. Arterioscler. Thromb. Vasc. Biol.. 27(10):2135-2141.
Abstract
OBJECTIVE: The role of ischemia in collateral vessel development (arteriogenesis) is a contentious issue that cannot be addressed using mammalian models. To investigate this, we developed models of arteriogenesis using the zebrafish embryo, which gains sufficient oxygenation via diffusion to prevent ischemia in response to arterial occlusion. METHODS AND RESULTS: We studied gridlock mutant embryos that suffer a permanently occluded aorta and show that these restore aortic blood flow by collateral vessels. We phenocopied gridlock mutants by laser-induced proximal aortic occlusion in transgenic Fli1:eGFP/GATA1:dsRED embryos. Serial imaging showed these restore aortic blood flow via collateral vessels by recruitment of preexisting endothelium in a manner similar to gridlocks. Collateral aortic blood flow in gridlock mutants was dependent on both nitric oxide and myeloid cells. Confocal microscopy of transgenic gridlock/Fli1:eGFP mutants demonstrated no aberrant angiogenic response to the aortic occlusion. qPCR of HIF1alpha expression confirmed the absence of hypoxia in this model system. CONCLUSIONS: We conclude that NO and myeloid cell-dependent collateral vessel development is an evolutionarily ancient response to arterial occlusion and is able to proceed in the absence of ischemia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping