PUBLICATION
CNS-derived glia ensheath peripheral nerves and mediate motor root development
- Authors
- Kucenas, S., Takada, N., Park, H.C., Woodruff, E., Broadie, K., and Appel, B.
- ID
- ZDB-PUB-080124-9
- Date
- 2008
- Source
- Nature Neuroscience 11(2): 143-151 (Journal)
- Registered Authors
- Appel, Bruce, Kucenas, Sarah, Park, Hae-Chul, Takada, Norio
- Keywords
- none
- MeSH Terms
-
- Axons/physiology*
- Spinal Cord/cytology
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Microscopy, Electron, Transmission
- Veratrum Alkaloids/pharmacology
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Zebrafish Proteins/genetics
- Motor Neurons/physiology*
- Gene Expression Regulation, Developmental/physiology
- Zonula Occludens-1 Protein
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Schwann Cells/physiology
- Schwann Cells/ultrastructure
- Animals
- Morpholines/pharmacology
- Animals, Genetically Modified
- Cell Differentiation
- Fishes
- Peripheral Nerves/cytology
- Peripheral Nerves/physiology*
- Neuroglia/physiology*
- Neuroglia/ultrastructure
- Embryo, Nonmammalian
- Phosphoproteins/genetics
- Phosphoproteins/metabolism
- Central Nervous System/cytology*
- Myelin Sheath/physiology*
- Myelin Sheath/ultrastructure
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- PubMed
- 18176560 Full text @ Nat. Neurosci.
Citation
Kucenas, S., Takada, N., Park, H.C., Woodruff, E., Broadie, K., and Appel, B. (2008) CNS-derived glia ensheath peripheral nerves and mediate motor root development. Nature Neuroscience. 11(2):143-151.
Abstract
Motor function requires that motor axons extend from the spinal cord at regular intervals and that they are myelinated by Schwann cells. Little attention has been given to another cellular structure, the perineurium, which ensheaths the motor nerve, forming a flexible, protective barrier. Consequently, the origin of perineurial cells and their roles in motor nerve formation are poorly understood. Using time-lapse imaging in zebrafish, we show that perineurial cells are born in the CNS, arising as ventral spinal-cord glia before migrating into the periphery. In embryos lacking perineurial glia, motor neurons inappropriately migrated outside of the spinal cord and had aberrant axonal projections, indicating that perineurial glia carry out barrier and guidance functions at motor axon exit points. Additionally, reciprocal signaling between perineurial glia and Schwann cells was necessary for motor nerve ensheathment by both cell types. These insights reveal a new class of CNS-born glia that critically contributes to motor nerve development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping