PUBLICATION
Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype
- Authors
- Pézeron, G., Lambert, G., Dickmeis, T., Strähle, U., Rosa, F.M., and Mourrain, P.
- ID
- ZDB-PUB-080218-4
- Date
- 2008
- Source
- PLoS One 3(1): e1434 (Journal)
- Registered Authors
- Dickmeis, Thomas, Lambert, Guillaume, Mourrain, Philippe, Pézeron, Guillaume, Rosa, Frederic, Strähle, Uwe
- Keywords
- Embryos, Zebrafish, Guanosine triphosphatase, Phenotypes, Messenger RNA, Endoderm, Phosphorylation, SMAD signaling
- MeSH Terms
-
- Animals
- Reverse Transcriptase Polymerase Chain Reaction
- Smad2 Protein/metabolism
- Zebrafish
- Phenotype
- Mutation*
- Monomeric GTP-Binding Proteins/genetics*
- Transcription Factors/genetics*
- Mutagenesis
- Zebrafish Proteins/genetics*
- Homeodomain Proteins/genetics*
- DNA Primers
- Base Sequence
- Phosphorylation
- PubMed
- 18197245 Full text @ PLoS One
Citation
Pézeron, G., Lambert, G., Dickmeis, T., Strähle, U., Rosa, F.M., and Mourrain, P. (2008) Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype. PLoS One. 3(1):e1434.
Abstract
The EGF-CFC factor Oep/Cripto1/Frl1 has been implicated in embryogenesis and several human cancers. During vertebrate development, Oep/Cripto1/Frl1 has been shown to act as an essential coreceptor in the TGFbeta/Nodal pathway, which is crucial for germ layer formation. Although studies in cell cultures suggest that Oep/Cripto1/Frl1 is also implicated in other pathways, in vivo it is solely regarded as a Nodal coreceptor. We have found that Rasl11b, a small GTPase belonging to a Ras subfamily of putative tumor suppressor genes, modulates Oep function in zebrafish independently of the Nodal pathway. rasl11b down regulation partially rescues endodermal and prechordal plate defects of zygotic oep(-/-) mutants (Zoep). Rasl11b inhibitory action was only observed in oep-deficient backgrounds, suggesting that normal oep expression prevents Rasl11b function. Surprisingly, rasl11b down regulation does not rescue mesendodermal defects in other Nodal pathway mutants, nor does it influence the phosphorylation state of the downstream effector Smad2. Thus, Rasl11b modifies the effect of Oep on mesendoderm development independently of the main known Oep output: the Nodal signaling pathway. This data suggests a new branch of Oep signaling that has implications for germ layer development, as well as for studies of Oep/Frl1/Cripto1 dysfunction, such as that found in tumors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping