PUBLICATION
Endothelial Signals Modulate Hepatocyte Apicobasal Polarization in Zebrafish
- Authors
- Sakaguchi, T.F., Sadler, K.C., Crosnier, C., and Stainier, D.Y.
- ID
- ZDB-PUB-081029-18
- Date
- 2008
- Source
- Current biology : CB 18(20): 1565-1571 (Journal)
- Registered Authors
- Sakaguchi, Takuya, Stainier, Didier
- Keywords
- CELLBIO, DEVBIO
- MeSH Terms
-
- Liver/cytology
- Liver/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/metabolism
- Gene Expression Regulation, Developmental
- Hepatocytes/metabolism*
- Cell Movement
- Animals
- Organogenesis
- Cell Membrane/metabolism
- Endothelial Cells/metabolism*
- Cell Polarity*
- Membrane Glycoproteins/metabolism
- Activated-Leukocyte Cell Adhesion Molecule/metabolism
- PubMed
- 18951027 Full text @ Curr. Biol.
Citation
Sakaguchi, T.F., Sadler, K.C., Crosnier, C., and Stainier, D.Y. (2008) Endothelial Signals Modulate Hepatocyte Apicobasal Polarization in Zebrafish. Current biology : CB. 18(20):1565-1571.
Abstract
Emerging evidence indicates that paracrine signals from endothelial cells play a role in tissue differentiation and organ formation [1-3]. Here, we identify a novel role for endothelial cells in modulating hepatocyte polarization during liver organogenesis. We find that in zebrafish, the apical domain of the hepatocytes predicts the location of the intrahepatic biliary network. The refinement of hepatocyte polarization coincides with the invasion of endothelial cells into the liver, and these endothelial cells migrate along the maturing basal surface of the hepatocytes. Using genetic, pharmacological, and transplantation experiments, we provide evidence that endothelial cells influence the polarization of the adjacent hepatocytes. This influence of endothelial cells on hepatocytes is mediated at least in part by the cell-surface protein Heart of glass and contributes to the establishment of coordinately aligned hepatocyte apical membranes and evenly spaced intrahepatic conduits.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping