PUBLICATION
FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1
- Authors
- Hong, S.K., and Dawid, I.B.
- ID
- ZDB-PUB-090204-7
- Date
- 2009
- Source
- Proceedings of the National Academy of Sciences of the United States of America 106(7): 2230-2235 (Journal)
- Registered Authors
- Dawid, Igor B., Hong, Sung-Kook
- Keywords
- ciliogenesis, Kupffer's vesicle, laterality
- MeSH Terms
-
- Phenotype
- Body Patterning*
- Fibroblast Growth Factors/metabolism*
- Models, Biological
- Humans
- Two-Hybrid System Techniques
- Cell Line
- Animals
- Carrier Proteins/genetics
- Carrier Proteins/physiology*
- Zebrafish
- Developmental Biology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology
- In Situ Hybridization
- Gene Expression Regulation, Developmental*
- Immediate-Early Proteins/genetics
- Immediate-Early Proteins/physiology*
- RNA, Messenger/metabolism
- PubMed
- 19164561 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Hong, S.K., and Dawid, I.B. (2009) FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1. Proceedings of the National Academy of Sciences of the United States of America. 106(7):2230-2235.
Abstract
Establishment of left-right asymmetry in vertebrates requires nodal, Wnt-PCP and FGF signaling and involves ciliogenesis in a laterality organ. Effector genes through which FGF signaling affects laterality have not been described. We isolated the zebrafish ier2 and fibp1 genes as FGF target genes and show that their protein products interact. Knock down of these factors interferes with establishment of organ laterality and causes defective cilia formation in Kupffer's Vesicle, the zebrafish laterality organ. Cilia are also lost after suppression of FGF8, but can be rescued by injection of ier2 and fibp1 mRNA. We conclude that Ier2 and Fibp1 mediate FGF signaling in ciliogenesis in Kupffer's Vesicle and in the establishment of laterality in the zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping