PUBLICATION
Apical polarity protein PrkCi is necessary for maintenance of spinal cord precursors in zebrafish
- Authors
- Roberts, R.K., and Appel, B.
- ID
- ZDB-PUB-090526-13
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(7): 1638-1648 (Journal)
- Registered Authors
- Appel, Bruce, Roberts, Randy
- Keywords
- neural precursors, zebrafish, oligodendrocytes, fate specification
- MeSH Terms
-
- Cell Proliferation
- Embryo, Nonmammalian
- Motor Neurons/physiology
- Cell Polarity/genetics*
- Zebrafish/embryology*
- Zebrafish/genetics
- Spinal Cord/embryology
- Spinal Cord/metabolism
- Spinal Cord/physiology*
- Protein Kinase C/genetics
- Protein Kinase C/physiology*
- Stem Cells/metabolism
- Stem Cells/physiology*
- Animals, Genetically Modified
- Adherens Junctions/genetics
- Adherens Junctions/physiology
- Animals
- Isoenzymes/genetics
- Isoenzymes/physiology*
- Cell Differentiation/genetics
- Neuroepithelial Cells/metabolism
- Neuroepithelial Cells/physiology
- PubMed
- 19449304 Full text @ Dev. Dyn.
Citation
Roberts, R.K., and Appel, B. (2009) Apical polarity protein PrkCi is necessary for maintenance of spinal cord precursors in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(7):1638-1648.
Abstract
During development, neural precursors divide to produce new precursors and cells that differentiate as neurons and glia. In Drosophila, apicobasal polarity and orientation of the mitotic spindle play important roles in specifying the progeny of neural precursors for different fates. We examined orientation of zebrafish spinal cord precursors using time-lapse imaging and tested the function of protein kinase C, iota (PrkCi), a member of the Par complex of proteins necessary for apicobasal polarity in the nervous system. We found that nearly all precursors divide within the plane of the neuroepithelium of wild-type embryos even when they must produce cells that have different fates. In the absence of PrkCi function, neural precursor divisions become oblique during late embryogenesis and excess oligodendrocytes form concomitant with loss of dividing cells. We conclude that PrkCi function and planar divisions are necessary for asymmetric, self-renewing division of spinal cord precursors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping