PUBLICATION
Characterization of Zebrafish Larval Inflammatory Macrophages
- Authors
- Mathias, J.R., Dodd, M.E., Walters, K.B., Yoo, S.K., Ranheim, E.A., and Huttenlocher, A.
- ID
- ZDB-PUB-090727-9
- Date
- 2009
- Source
- Developmental and comparative immunology 33(11): 1212-1217 (Journal)
- Registered Authors
- Huttenlocher, Anna, Mathias, Jonathan
- Keywords
- Zebrafish, Inflammation, Macrophage, Neutrophil, Phagocytosis
- MeSH Terms
-
- Animals
- Cell Separation
- Zebrafish/immunology*
- Flow Cytometry
- Microfilament Proteins/genetics
- Microfilament Proteins/immunology
- Microfilament Proteins/metabolism*
- Antigens, Differentiation/genetics
- Antigens, Differentiation/immunology
- Antigens, Differentiation/metabolism*
- Inflammation
- Receptor, Macrophage Colony-Stimulating Factor/genetics
- Receptor, Macrophage Colony-Stimulating Factor/immunology
- Receptor, Macrophage Colony-Stimulating Factor/metabolism*
- Peroxidase/metabolism
- Phagocytosis
- Cell Movement/immunology
- Pseudopodia/immunology
- Macrophages/immunology
- Macrophages/metabolism*
- Macrophages/pathology
- Animals, Genetically Modified
- Membrane Glycoproteins/genetics
- Membrane Glycoproteins/immunology
- Membrane Glycoproteins/metabolism*
- Larva/immunology
- PubMed
- 19619578 Full text @ Dev. Comp. Immunol.
Citation
Mathias, J.R., Dodd, M.E., Walters, K.B., Yoo, S.K., Ranheim, E.A., and Huttenlocher, A. (2009) Characterization of Zebrafish Larval Inflammatory Macrophages. Developmental and comparative immunology. 33(11):1212-1217.
Abstract
Zebrafish have emerged as a powerful model system to study leukocyte recruitment and inflammation. Here we characterize the morphology and function of inflammatory macrophages in zebrafish larvae. These macrophages can be distinguished from neutrophils by immunolabeling of L-Plastin without MPO co-expression and by an elongated morphology. Live imaging of transgenic zMPO:GFP larvae demonstrate that GFP(lo) macrophages migrate to wounds by extension of thin pseudopods and carry out phagocytosis of tissue debris, and FACS analysis of leukocyte markers indicates expression of CSF1R in these macrophages. These findings identify distinct functional and morphological characteristics of inflammatory macrophages in zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping