PUBLICATION
Zebrafish CiA interneurons are late-born primary neurons
- Authors
- Yeo, S.Y.
- ID
- ZDB-PUB-091023-14
- Date
- 2009
- Source
- Neuroscience letters 466(3): 131-134 (Journal)
- Registered Authors
- Yeo, Sang-Yeob
- Keywords
- Notch, Zebrafish, Pax2, CiA, Primary neurons, Late-born neurons, Mind bomb, CoPA
- MeSH Terms
-
- Animals
- Spinal Cord/cytology
- Spinal Cord/embryology
- Spinal Cord/metabolism
- Xenopus
- Embryo, Nonmammalian
- Immunohistochemistry
- Zebrafish/embryology*
- Zebrafish/metabolism
- Interneurons/cytology*
- Interneurons/metabolism
- Gene Knockdown Techniques
- Ubiquitin-Protein Ligases/genetics
- Receptors, Notch/genetics
- Neurons/cytology*
- Neurons/metabolism
- Mutation
- RNA, Messenger/genetics
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- PAX2 Transcription Factor/biosynthesis
- PubMed
- 19800937 Full text @ Neurosci. Lett.
Citation
Yeo, S.Y. (2009) Zebrafish CiA interneurons are late-born primary neurons. Neuroscience letters. 466(3):131-134.
Abstract
Pax2 is a neural-related transcription factor downstream of Notch signaling and is expressed in the developing spinal cord of zebrafish, including in CiA interneurons. However, the characteristics of pax2-positive neurons are largely unknown. The goal of this study was to characterize Pax2-positive neurons by examining their expression in embryos in which Notch function had been knocked down by mutation or injection of a morpholino or mRNA. I found that Pax2-positive CiA interneurons were late-differentiating primary neurons. pax2.1 was expressed in CoPA commissural neurons and CiA interneurons at 26 hpf. The number of pax2.1-positive cells increased in mind bomb mutant embryos or embryos injected with Su(H)1-MO, but not in cells injected with Xenopus Delta or Delta(stu) mRNA. These observations imply that Notch signaling plays a role in regulating the number of CiA neurons by preventing uncommitted precursors from acquiring a neuronal fate during vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping