PUBLICATION
Positional cloning of a novel allele of zebrafish cloche mutant
- Authors
- Ma, N., Huo, Z.J., Yan, G., Huang, H.H., Luo, S.Q., and Zhang, W.Q.
- ID
- ZDB-PUB-100330-17
- Date
- 2010
- Source
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University 30(3): 458-462 (Journal)
- Registered Authors
- Huang, Honghui, Ma, Ning
- Keywords
- none
- MeSH Terms
-
- Zebrafish Proteins/genetics*
- Alleles
- Male
- Mutation*
- Genetic Complementation Test
- Animals
- Chromosome Mapping
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Ethylnitrosourea/toxicity
- Muramidase/genetics*
- Zebrafish/embryology
- Zebrafish/genetics*
- Cloning, Molecular
- PubMed
- 20335109
Citation
Ma, N., Huo, Z.J., Yan, G., Huang, H.H., Luo, S.Q., and Zhang, W.Q. (2010) Positional cloning of a novel allele of zebrafish cloche mutant. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 30(3):458-462.
Abstract
OBJECTIVE: To perform the genetic idencification of cloche(172) mutant zebrafish. METHODS: The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to treat the AB stain male fish. Large-scale forward genetic screening was carried out to search for lyC-deficient zebrafish mutant by WISH. The morphology changes of the embryos at 3 days postfertilization (3dpf) stage were observed and the cloche(172) gene was identified by mapping and complementation test. RESULTS: We selected 4 lyC-deficient zebrafish by WISH. cloche(172) mutant showed morphological changes similar to cloche mutant in 3dpf stage. One fourth of the embryos showed cloche phenotype as found in complementation test, and the cloche(172) gene was mapped on the telomere of zebrafish 13 chromosome where cloche gene was located. Numerous red blood cells were observed in the cloche(172) mutant, while only a few cells were found in the cloche mutant in the tail region by o-dianisdine staining. CONCLUSION: cloche(172) gene which is responsible for the phenotype of cloche mutant may be a novel point mutation allele of the cloche mutant.
Errata / Notes
Article in Chinese.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping