PUBLICATION
Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons
- Authors
- Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H.
- ID
- ZDB-PUB-101201-20
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(1): 9-22 (Journal)
- Registered Authors
- Nakayama, Ryoko, Okamoto, Hitoshi, Sato, Miki, Shoji, Wataru, Tanaka, Hideomi
- Keywords
- Islet1, Dpysl3, CRMP4, semaphorin, Rohon-Beard neuron, axon guidance, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Nerve Tissue Proteins/physiology*
- Axons/metabolism
- Axons/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- Genetic Loci
- Amino Acid Sequence
- LIM-Homeodomain Proteins
- Models, Biological
- Molecular Sequence Data
- Motor Neurons/metabolism
- Motor Neurons/physiology
- Sensory Receptor Cells/metabolism
- Sensory Receptor Cells/physiology*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/physiology*
- Gene Knockdown Techniques
- Base Sequence
- Animals, Genetically Modified
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology
- Transcription Factors
- Mutation/physiology
- Spinal Nerves/abnormalities
- Spinal Nerves/embryology
- Spinal Nerves/metabolism
- Sequence Homology
- PubMed
- 21108318 Full text @ Dev. Dyn.
Citation
Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H. (2011) Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(1):9-22.
Abstract
We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon-Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down-regulation of the expression of dihydropyrimidinase-like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)-mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping