PUBLICATION
Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation
- Authors
- Taneda, T., Zhu, W., Cao, Q., Watanabe, H., Yamaguchi, Y., Handa, H., and Wada, T.
- ID
- ZDB-PUB-110110-24
- Date
- 2011
- Source
- Genes to cells : devoted to molecular & cellular mechanisms 16(2): 231-242 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE6127
- MeSH Terms
-
- Erythropoiesis/genetics*
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Gene Expression Regulation, Developmental*
- Hematopoietic Stem Cells/metabolism
- GATA1 Transcription Factor/genetics*
- Molecular Sequence Data
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- Amino Acid Sequence
- Animals
- PubMed
- 21205096 Full text @ Genes Cells
Citation
Taneda, T., Zhu, W., Cao, Q., Watanabe, H., Yamaguchi, Y., Handa, H., and Wada, T. (2011) Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation. Genes to cells : devoted to molecular & cellular mechanisms. 16(2):231-242.
Abstract
Recent studies have showed that transcription elongation factors regulate early development. Foggy/Spt5 is a subunit of DRB sensitivity-inducing factor, which negatively and positively regulates transcription elongation. Here, we report that the positive function of Foggy/Spt5 is required for gata1 expression during zebrafish embryonic hematopoiesis. Antisense morpholino oligonucleotide (MO)-mediated knockdown of foggy/spt5 has led to a reduction in the expression of gata1 and the gata1 target genes alas2 and hbae3 and inhibited proper hemoglobin production. By contrast, expression of hematopoietic stem cell and endothelial markers, including scl, lmo2, gata2, fli-1, and flk-1, and expression of biklf, whose product directs gata1 expression via its direct binding to the gata1 promoter, were unaltered, suggesting that gata1 is a functionally important target gene of Foggy/Spt5. The MO-mediated gata1 repression was relieved by forced expression of wild-type foggy/spt5, but not by a mutant lacking the positive function. Therefore, this study provides evidence that Foggy/Spt5 plays an important role in gata1 gene expression and erythropoiesis through its transcriptional activation domain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping