PUBLICATION
Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development
- Authors
- Poulain, M., and Ober, E.A.
- ID
- ZDB-PUB-110721-9
- Date
- 2011
- Source
- Development (Cambridge, England) 138(16): 3557-68 (Journal)
- Registered Authors
- Ober, Elke, Poulain, Morgane
- Keywords
- wnt signalling, liver, swim bladder, endoderm patterning, organogenesis, zebrafish
- MeSH Terms
-
- Animals
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Digestive System/cytology
- Digestive System/embryology*
- Digestive System/metabolism*
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Gene Expression Regulation, Developmental
- Wnt2 Protein/genetics
- Wnt2 Protein/metabolism*
- Frizzled Receptors/genetics
- Frizzled Receptors/metabolism
- Cell Proliferation
- PubMed
- 21771809 Full text @ Development
Citation
Poulain, M., and Ober, E.A. (2011) Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development (Cambridge, England). 138(16):3557-68.
Abstract
The vertebrate liver, pancreas and lung arise in close proximity from the multipotent foregut endoderm. Tissue-explant
experiments uncovered instructive signals emanating from the neighbouring lateral plate mesoderm, directing the endoderm
towards specific organ fates. This suggested that an intricate network of signals is required to control the specification and
differentiation of each organ. Here, we show that sequential functions of Wnt2bb and Wnt2 control liver specification and
proliferation in zebrafish. Their combined specific activities are essential for liver specification, as their loss of function causes liver
agenesis. Conversely, excess wnt2bb or wnt2 induces ectopic liver tissue at the expense of pancreatic and anterior intestinal
tissues, revealing the competence of intestinal endoderm to respond to hepatogenic signals. Epistasis experiments revealed that
the receptor frizzled homolog 5 (fzd5) mediates part of the broader hepatic competence of the alimentary canal. fzd5 is required
for early liver formation and interacts genetically with wnt2 as well as wnt2bb. In addition, lack of both ligands causes agenesis
of the swim bladder, the structural homolog of the mammalian lung. Thus, tightly regulated spatiotemporal expression of
wnt2bb, wnt2 and fzd5 is central to coordinating early liver, pancreas and swim bladder development from a multipotent foregut
endoderm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping