PUBLICATION
Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging
- Authors
- Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y.
- ID
- ZDB-PUB-120705-28
- Date
- 2012
- Source
- Optics letters 37(13): 2490-2492 (Journal)
- Registered Authors
- Wen, Zilong
- Keywords
- none
- MeSH Terms
-
- Spectrometry, Fluorescence/methods*
- Flow Cytometry/methods*
- Photons*
- Blood Circulation
- Leukocytes/cytology
- Erythrocytes/cytology
- Zebrafish*/physiology
- Animals
- Molecular Imaging/methods*
- PubMed
- 22743431 Full text @ Opt. Lett.
Citation
Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y. (2012) Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging. Optics letters. 37(13):2490-2492.
Abstract
We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping