PUBLICATION
Ruthenium-Caged Antisense Morpholinos for Regulating Gene Expression in Zebrafish Embryos
- Authors
- Griepenburg, J.C., Rapp, T.L., Carroll, P.J., Eberwine, J., Dmochowski, I.J.
- ID
- ZDB-PUB-150530-3
- Date
- 2015
- Source
- Chemical science 6: 2342-2346 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 26023327 Full text @ Chem Sci
Citation
Griepenburg, J.C., Rapp, T.L., Carroll, P.J., Eberwine, J., Dmochowski, I.J. (2015) Ruthenium-Caged Antisense Morpholinos for Regulating Gene Expression in Zebrafish Embryos. Chemical science. 6:2342-2346.
Abstract
Photochemical approaches afford high spatiotemporal control over molecular structure and function, for broad applications in materials and biological science. Here, we present the first example of a visible light responsive ruthenium-based photolinker, Ru(bipyridine)2(3-ethynylpyridine)2 (RuBEP), which was reacted stoichiometrically with a 25mer DNA or morpholino (MO) oligonucleotide functionalized with 3' and 5' terminal azides, via Cu(I)-mediated [3+2] Huisgen cycloaddition reactions. RuBEP-caged circular morpholinos (Ru-MOs) targeting two early developmental zebrafish genes, chordin and notail, were synthesized and tested in vivo. One-cell-stage zebrafish embryos microinjected with Ru-MO and incubated in the dark for 24 h developed normally, consistent with caging, whereas irradiation at 450 nm dissociated one 3-ethynylpyridine ligand (ϕ = 0.33) and uncaged the MO to achieve gene knockdown. As demonstrated, Ru photolinkers provide a versatile method for controlling structure and function of biopolymers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping