PUBLICATION
The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish
- Authors
- Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M.
- ID
- ZDB-PUB-150808-10
- Date
- 2015
- Source
- Mechanisms of Development 138 Pt 3: 328-35 (Journal)
- Registered Authors
- Vasilyev, Aleksandr, Yuan, Shipeng, Zhang, Jiaojiao
- Keywords
- Taz, kidney cysts, patterning, pronephros, zebrafish
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Body Patterning/drug effects
- Body Patterning/genetics
- Body Patterning/physiology
- Mesoderm/cytology
- Mesoderm/embryology
- Mesoderm/metabolism
- Animals, Genetically Modified
- Animals
- Tretinoin/metabolism
- Tretinoin/pharmacology
- Pronephros/cytology
- Pronephros/embryology*
- Pronephros/metabolism
- Intracellular Signaling Peptides and Proteins/antagonists & inhibitors
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Cell Count
- Kidney Tubules/cytology
- Kidney Tubules/embryology*
- Kidney Tubules/metabolism
- Gene Knockdown Techniques
- PubMed
- 26248207 Full text @ Mech. Dev.
Citation
Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M. (2015) The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish. Mechanisms of Development. 138 Pt 3:328-35.
Abstract
The zebrafish pronephric tubule consists of a proximal and distal segments and a collecting duct. The proximal segment is subdivided into the neck, proximal convoluted (PCT) and proximal straight (PST) tubule segments. The distal segment consists of a distal-early (DE) and late (DL) segments. How the proximal and distal segments develop along the anteroposterior axis is poorly understood. Here we show that knockdown of taz in zebrafish caused shortening and a significant reduction in number of principal cells of the PST-DE segment, and proximalization of the pronephric tubule in 24 hpf embryos. RA treatment expanded the pronephric proximal domain in normal embryos as in taz morphants, an effect that was further enhanced upon exposure of taz morphants to RA. The early pronephric defects in 24 hpf taz morphants led to failure of anterior pronephric tubule migration and convolution, and to PCT dilation and cyst formation in older embryos. In situ hybridization showed weak and transient expression of taz at the bud stage in intermediate mesoderm, the source of pronephric progenitors. The present findings show that Taz is required in anteroposterior patterning of the pronephric progenitor domain in intermediate mesoderm, acting in part by regulating RA signaling in pronephric progenitor field in intermediate mesoderm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping