PUBLICATION
The aPKC/Par3/Par6 polarity complex and membrane order are functionally inter-dependent in epithelia during vertebrate organogenesis
- Authors
- Abu-Siniyeh, A., Owen, D.M., Benzing, C., Rinkwitz, S., Becker, T.S., Majumdar, A., Gaus, K.
- ID
- ZDB-PUB-151013-4
- Date
- 2016
- Source
- Traffic (Copenhagen, Denmark) 17(1): 66-79 (Journal)
- Registered Authors
- Abu-Siniyeh, Ahmed, Becker, Thomas S., Majumdar, Arindam, Rinkwitz, Silke
- Keywords
- apical and basolateral membranes, epithelial cell, lipid phases, membrane organisation, polarity proteins, zebrafish larvae
- MeSH Terms
-
- Membrane Proteins
- Animals
- Zebrafish Proteins/metabolism*
- Organogenesis*
- Cell Membrane/metabolism*
- Carrier Proteins/metabolism*
- Protein Kinase C/metabolism*
- Cholesterol/metabolism
- Zebrafish
- Epithelium/embryology
- Epithelium/metabolism*
- PubMed
- 26456025 Full text @ Traffic
Citation
Abu-Siniyeh, A., Owen, D.M., Benzing, C., Rinkwitz, S., Becker, T.S., Majumdar, A., Gaus, K. (2016) The aPKC/Par3/Par6 polarity complex and membrane order are functionally inter-dependent in epithelia during vertebrate organogenesis. Traffic (Copenhagen, Denmark). 17(1):66-79.
Abstract
The differential distribution of lipids between apical and basolateral membranes is necessary for many epithelial cell functions, but how this characteristic membrane organization is integrated within the polarity network during ductal organ development is poorly understood. Here we quantified membrane order in the gut, kidney, and liver ductal epithelia in zebrafish larvae at 3-11 days post fertilization (dpf) with Laurdan 2-photon microscopy. We then applied a combination of laurdan imaging, antisense knockdown, and analysis of polarity markers to understand the relationship between membrane order and apical basal polarity. We found a reciprocal relationship between membrane order and the cell polarity network. Reducing membrane condensation by exogenously added oxysterol or depletion of cholesterol reduced apical targeting of the polarity protein, aPKC. Conversely, using morpholino knockdown in zebrafish, we found that membrane order was dependent upon the Crb3 and Par3 polarity protein expression in ductal epithelia. Hence our data suggest that the biophysical property of membrane lipid packing is a regulatory element in apical basal polarity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping