PUBLICATION
Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
- Authors
- Icha, J., Kunath, C., Rocha-Martins, M., Norden, C.
- ID
- ZDB-PUB-161105-15
- Date
- 2016
- Source
- The Journal of cell biology 215: 259-275 (Journal)
- Registered Authors
- Icha, Jaroslav, Norden, Caren
- Keywords
- none
- MeSH Terms
-
- Cell Nucleus/metabolism
- Cell Movement*
- Kinetics
- Actin-Related Protein 2-3 Complex/metabolism
- Embryo, Nonmammalian/cytology
- Animals
- Retinal Ganglion Cells/cytology*
- Models, Biological
- Stem Cells/cytology
- Stem Cells/metabolism
- Organelles/metabolism
- Cell Differentiation
- Cell Survival
- Microtubules/metabolism
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 27810916 Full text @ J. Cell Biol.
Citation
Icha, J., Kunath, C., Rocha-Martins, M., Norden, C. (2016) Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina. The Journal of cell biology. 215:259-275.
Abstract
The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping