PUBLICATION
The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation
- Authors
- Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P.
- ID
- ZDB-PUB-170222-18
- Date
- 2017
- Source
- Developmental Cell 40(4): 354-366.e4 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Morita, Hitoshi
- Keywords
- active fluid description of tissue spreading, gastrulation, interfacial tension, radial cell intercalation, surface cell expansion, zebrafish
- MeSH Terms
-
- Cell Movement
- Zebrafish/embryology*
- Zebrafish/physiology*
- Computer Simulation
- Blastoderm/cytology
- Blastoderm/metabolism
- Cell Count
- Stress, Physiological
- Morphogenesis*
- Cell Proliferation
- Embryo, Nonmammalian/cytology
- Gastrulation*
- Surface Tension
- Biophysical Phenomena*
- Animals
- Cell Communication
- PubMed
- 28216382 Full text @ Dev. Cell
Citation
Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P. (2017) The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation. Developmental Cell. 40(4):354-366.e4.
Abstract
Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during "doming," when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping