PUBLICATION
Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model
- Authors
- Yang, Z., Chen, S., Xue, S., Li, X., Sun, Z., Yang, Y., Hu, X., Geng, T., Cui, H.
- ID
- ZDB-PUB-180924-5
- Date
- 2018
- Source
- Biotechnology Letters 40(11-12): 1507-1518 (Journal)
- Registered Authors
- Keywords
- CRISPR/Cas9, Embryonic development, Genomic editing, Spinal abnormality, Zebrafish
- MeSH Terms
-
- Gene Editing/methods*
- Male
- Zebrafish/genetics*
- Gene Knockout Techniques
- Female
- Endogenous Retroviruses/genetics*
- Animals, Genetically Modified/genetics*
- CRISPR-Cas Systems/genetics*
- Promoter Regions, Genetic/genetics
- Animals
- PubMed
- 30244429 Full text @ Biotechnol. Lett.
Citation
Yang, Z., Chen, S., Xue, S., Li, X., Sun, Z., Yang, Y., Hu, X., Geng, T., Cui, H. (2018) Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model. Biotechnology Letters. 40(11-12):1507-1518.
Abstract
Objectives To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish.
Results Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish can improve the efficiency of genome editing significantly. At the same time, we first observed that the phenotype of vertebral malformation in early embryonic development of zebrafish after ZFERV knockout.
Conclusions The transgenic zebrafish with expressed Cas9, is more efficient in genome editing. And the results of ZFERV knockout indicated that ERV may affect the vertebral development by Notch1/Delta D signal pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping