PUBLICATION
In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone
- Authors
- Cox, B.D., De Simone, A., Tornini, V.A., Singh, S.P., Di Talia, S., Poss, K.D.
- ID
- ZDB-PUB-181207-6
- Date
- 2018
- Source
- Current biology : CB 28(24): 3937-3947.e4 (Journal)
- Registered Authors
- Cox, Ben, Poss, Kenneth D., Singh, Sumeet Pal, Tornini, Valerie A.
- Keywords
- bone, differentiation, fibroblast growth factor, live imaging, osteoblast, proliferation, regeneration, scale, zebrafish
- MeSH Terms
-
- Animals
- Bone Regeneration/physiology*
- Cell Differentiation
- Osteoblasts/physiology*
- Animals, Genetically Modified
- Zebrafish/physiology*
- Cell Division
- Optical Imaging/methods*
- Single-Cell Analysis/methods*
- PubMed
- 30503623 Full text @ Curr. Biol.
Citation
Cox, B.D., De Simone, A., Tornini, V.A., Singh, S.P., Di Talia, S., Poss, K.D. (2018) In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone. Current biology : CB. 28(24):3937-3947.e4.
Abstract
Osteoblasts are matrix-depositing cells that can divide and heal bone injuries. Their deep-tissue location and the slow progression of bone regeneration challenge attempts to capture osteoblast behaviors in live tissue at high spatiotemporal resolution. Here, we have developed an imaging platform to monitor and quantify individual and collective behaviors of osteoblasts in adult zebrafish scales, skeletal body armor discs that regenerate rapidly after loss. Using a panel of transgenic lines that visualize and manipulate osteoblasts, we find that a founder pool of osteoblasts emerges through de novo differentiation within one day of scale plucking. These osteoblasts undergo division events that are largely uniform in frequency and orientation to establish a primordium. Osteoblast proliferation dynamics diversify across the primordium by two days after injury, with cell divisions focused near, and with orientations parallel to, the scale periphery, occurring coincident with dynamic localization of fgf20a gene expression. In posterior scale regions, cell elongation events initiate in areas soon occupied by mineralized grooves called radii, beginning approximately 2 days post injury, with patterned osteoblast death events accompanying maturation of these radii. By imaging at single-cell resolution, we detail acquisition of spatiotemporally distinct cell division, motility, and death dynamics within a founder osteoblast pool as bone regenerates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping