PUBLICATION
Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury
- Authors
- Stoddard, M., Huang, C., Enyedi, B., Niethammer, P.
- ID
- ZDB-PUB-190113-15
- Date
- 2019
- Source
- Scientific Reports 9: 28 (Journal)
- Registered Authors
- Enyedi, Balázs, Huang, Cong, Niethammer, Philipp
- Keywords
- none
- MeSH Terms
-
- Anti-Infective Agents/administration & dosage
- Anti-Infective Agents/adverse effects
- Chemical and Drug Induced Liver Injury/pathology*
- Immunity, Innate*
- Animals
- Leukocytes/immunology*
- Oxidative Stress
- Animals, Genetically Modified
- Intravital Microscopy/methods*
- Metronidazole/administration & dosage
- Metronidazole/adverse effects
- Recombinant Proteins/metabolism
- Gene Expression
- Zebrafish
- Nitroreductases/metabolism
- Disease Models, Animal
- PubMed
- 30631093 Full text @ Sci. Rep.
Citation
Stoddard, M., Huang, C., Enyedi, B., Niethammer, P. (2019) Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury. Scientific Reports. 9:28.
Abstract
Studying early immune responses to organ damage in situ requires animal models amenable to intravital imaging. Here, we used transparent zebrafish larvae, a powerful animal model for innate immunity, to measure leukocyte recruitment to damaged livers. Bath application of metronidazole (Mtz) to fish expressing nitroreductase (NTR) under a liver-specific promoter damaged the organ within 24 hours causing oxidative stress, distorted liver morphology, accumulation of TUNEL-positive cells, and transcriptional upregulation of apoptotic and antioxidant genes. Inflammatory gene transcription in damaged hepatocytes was attenuated. In line with predominant apoptosis, macrophages were massively recruited into Mtz/NTR-damaged livers. By contrast, neutrophil infiltration was more variable and delayed, consistent with less abundant necrosis and an attenuated inflammatory capacity of damaged hepatocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping