PUBLICATION
Clusters of neuronal neurofascin prefigure the position of a subset of nodes of Ranvier along individual central nervous system axons in vivo
- Authors
- Vagionitis, S., Auer, F., Xiao, Y., Almeida, R.G., Lyons, D.A., Czopka, T.
- ID
- ZDB-PUB-220217-6
- Date
- 2022
- Source
- Cell Reports 38: 110366 (Journal)
- Registered Authors
- Almeida, Rafael, Auer, Franziska, Czopka, Tim, Lyons, David A., Vagionitis, Stavros, Xiao, Yan
- Keywords
- axon-oligodendrocyte interactions, central nervous system, myelination, node of Ranvier, zebrafish
- MeSH Terms
-
- Central Nervous System/metabolism*
- Oligodendroglia/metabolism
- Genes, Reporter
- Animals
- Axons/metabolism*
- Ranvier's Nodes/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Mutation/genetics
- Zebrafish/metabolism*
- Myelin Sheath/metabolism
- PubMed
- 35172135 Full text @ Cell Rep.
Citation
Vagionitis, S., Auer, F., Xiao, Y., Almeida, R.G., Lyons, D.A., Czopka, T. (2022) Clusters of neuronal neurofascin prefigure the position of a subset of nodes of Ranvier along individual central nervous system axons in vivo. Cell Reports. 38:110366.
Abstract
The spacing of nodes of Ranvier crucially affects conduction properties along myelinated axons. It is assumed that node position is primarily driven by growing myelin sheaths. Here, we reveal an additional mechanism of node positioning that is driven by the axon. Through longitudinal live imaging of node formation dynamics in the zebrafish central nervous system, we show that stable clusters of the cell adhesion molecule neurofascin a can accumulate at specific sites along axons prior to myelination. While some of these clusters are pushed into future node position by extending myelin sheaths, others are not and thus prefigure the position of where a mature node forms. Animals that lack full-length neurofascin a show increased internodal distances and less regular nodal spacing along single axons. Together, our data reveal the existence of an axonal mechanism to position nodes of Ranvier that does not depend on regulation by myelin sheath growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping