PUBLICATION
GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate
- Authors
- Song, M., Yuan, X., Racioppi, C., Leslie, M., Stutt, N., Aleksandrova, A., Christiaen, L., Wilson, M.D., Scott, I.C.
- ID
- ZDB-PUB-220312-5
- Date
- 2022
- Source
- Science advances 8: eabg0834 (Journal)
- Registered Authors
- Aleksandrova, Anastasiia, Leslie, Meaghan, Scott, Ian, Song, Mengyi, Yuan, Xuefei
- Keywords
- none
- MeSH Terms
-
- GATA4 Transcription Factor*/genetics
- GATA4 Transcription Factor*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- GATA5 Transcription Factor/genetics
- GATA5 Transcription Factor/metabolism
- Mesoderm/metabolism
- Animals
- Gene Expression Regulation, Developmental
- PubMed
- 35275720 Full text @ Sci Adv
Citation
Song, M., Yuan, X., Racioppi, C., Leslie, M., Stutt, N., Aleksandrova, A., Christiaen, L., Wilson, M.D., Scott, I.C. (2022) GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate. Science advances. 8:eabg0834.
Abstract
GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping