PUBLICATION
Exposure to diclofenac alters thyroid hormone levels and transcription of genes involved in the hypothalamic-pituitary-thyroid axis in zebrafish embryos/larvae
- Authors
- Wang, H., Dong, F., Zhao, Y., Fu, S., Zhao, H., Liu, S., Zhang, W., Hu, F.
- ID
- ZDB-PUB-220331-8
- Date
- 2022
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 257: 109335 (Journal)
- Registered Authors
- Keywords
- Diclofenac, Embryonic development, Heartbeat, Thyroid disruption, Zebrafish
- MeSH Terms
-
- Thyroid Hormones
- Zebrafish*
- Diclofenac/toxicity
- Thyroid Gland
- Water Pollutants, Chemical*/toxicity
- Animals
- Larva/genetics
- PubMed
- 35351617 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Wang, H., Dong, F., Zhao, Y., Fu, S., Zhao, H., Liu, S., Zhang, W., Hu, F. (2022) Exposure to diclofenac alters thyroid hormone levels and transcription of genes involved in the hypothalamic-pituitary-thyroid axis in zebrafish embryos/larvae. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 257:109335.
Abstract
Diclofenac (DCF), one of typical non-steroidal anti-inflammatory drugs (NSAIDs), has been frequently detected in various environmental media. Nevertheless，the potential endocrine disrupting effects of DCF on fish were poorly understood. In the present study, zebrafish embryos/larvae were used as a model to evaluate the adverse effects of DCF on development and thyroid system. The results demonstrated that DCF only significantly decreased the heart rate at 72 h post-fertilization (hpf), exhibiting limited influence on the embryonic development of zebrafish. Treatment with DCF significantly reduced whole-body thyroxine (T4) levels, and changed transcriptional levels of several genes related to the hypothalamic-pituitary-thyroid (HPT) axis. These findings provide important information regarding to the mechanisms of DCF-induced developmental toxicity and thyroid disruption in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping