PUBLICATION
Alcam-a and Pdgfr-α are essential for the development of sclerotome-derived stromal cells that support hematopoiesis
- Authors
- Murayama, E., Vivier, C., Schmidt, A., Herbomel, P.
- ID
- ZDB-PUB-230302-41
- Date
- 2023
- Source
- Nature communications 14: 11711171 (Journal)
- Registered Authors
- Herbomel, Philippe, Murayama, Emi, Schmidt, Anne, Vivier, Catherine
- Keywords
- none
- MeSH Terms
-
- Receptor, Platelet-Derived Growth Factor alpha*
- Zebrafish
- Receptor Protein-Tyrosine Kinases
- Stromal Cells
- Mammals
- Animals
- Hematopoiesis
- Hematopoietic Stem Cells
- Activated-Leukocyte Cell Adhesion Molecule*
- PubMed
- 36859431 Full text @ Nat. Commun.
Citation
Murayama, E., Vivier, C., Schmidt, A., Herbomel, P. (2023) Alcam-a and Pdgfr-α are essential for the development of sclerotome-derived stromal cells that support hematopoiesis. Nature communications. 14:11711171.
Abstract
Mesenchymal stromal cells are essential components of hematopoietic stem and progenitor cell (HSPC) niches, regulating HSPC proliferation and fates. Their developmental origins are largely unknown. In zebrafish, we previously found that the stromal cells of the caudal hematopoietic tissue (CHT), a niche functionally homologous to the mammalian fetal liver, arise from the ventral part of caudal somites. We have now found that this ventral domain is the sclerotome, and that two markers of mammalian mesenchymal stem/stromal cells, Alcam and Pdgfr-α, are distinctively expressed there and instrumental for the emergence and migration of stromal cell progenitors, which in turn conditions the proper assembly of the vascular component of the CHT niche. Furthermore, we find that trunk somites are similarly dependent on Alcam and Pdgfr-α to produce mesenchymal cells that foster HSPC emergence from the aorta. Thus the sclerotome contributes essential stromal cells for each of the key steps of developmental hematopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping