PUBLICATION
wnt10a is required for zebrafish median fin fold maintenance and adult unpaired fin metamorphosis
- Authors
- Benard, E.L., Küçükaylak, I., Hatzold, J., Berendes, K.U.W., Carney, T.J., Beleggia, F., Hammerschmidt, M.
- ID
- ZDB-PUB-231024-51
- Date
- 2023
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 253(6): 566-592 (Journal)
- Registered Authors
- Benard, Erica L., Carney, Tom, Hammerschmidt, Matthias, Hatzold, Julia
- Keywords
- fin metamorphosis, median fin fold, tooth development, wnt10a, zebrafish
- MeSH Terms
-
- Animal Fins*/embryology
- Animal Fins*/growth & development
- Animal Fins*/metabolism
- Mutation
- Animals
- Wnt Proteins*/genetics
- Wnt Proteins*/metabolism
- Metamorphosis, Biological*/genetics
- Zebrafish*/embryology
- Zebrafish*/growth & development
- Gene Expression Regulation, Developmental
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 37870737 Full text @ Dev. Dyn.
Citation
Benard, E.L., Küçükaylak, I., Hatzold, J., Berendes, K.U.W., Carney, T.J., Beleggia, F., Hammerschmidt, M. (2023) wnt10a is required for zebrafish median fin fold maintenance and adult unpaired fin metamorphosis. Developmental Dynamics : an official publication of the American Association of Anatomists. 253(6):566-592.
Abstract
Background Mutations of human WNT10A are associated with odonto-ectodermal dysplasia syndromes. Here, we present analyses of wnt10a loss-of-function mutants in the zebrafish.
Results wnt10a mutant zebrafish embryos display impaired tooth development and a collapsing median fin fold (MFF). Rescue experiments show that wnt10a is essential for MFF maintenance both during embryogenesis and later metamorphosis. The MFF collapse could not be attributed to increased cell death or altered proliferation rates of MFF cell types. Rather, wnt10a mutants show reduced expression levels of dlx2a in distal-most MFF cells, followed by compromised expression of col1a1a and other extracellular matrix proteins encoding genes. Transmission electron microscopy analysis shows that although dermal MFF compartments of wnt10a mutants initially are of normal morphology, with regular collagenous actinotrichia, positioning of actinotrichia within the cleft of distal MFF cells becomes compromised, coinciding with actinotrichia shrinkage and MFF collapse.
Conclusions MFF collapse of wnt10a mutant zebrafish is likely caused by the loss of distal properties in the developing MFF, strikingly similar to the proposed molecular pathomechanisms underlying the teeth defects caused by the loss of Wnt10 in fish and mammals. In addition, it points to thus fur unknown mechanisms controlling the linear growth and stability of actinotrichia and their collagen fibrils.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping