PUBLICATION
Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis
- Authors
- da Silva Pescador, G., Baia Amaral, D., Varberg, J.M., Zhang, Y., Hao, Y., Florens, L., Bazzini, A.A.
- ID
- ZDB-PUB-240921-5
- Date
- 2024
- Source
- Cell Reports 43: 114769114769 (Journal)
- Registered Authors
- Keywords
- CP: Developmental biology, CP: Molecular biology, CRISPR-Cas13d, SLAM-seq, Zfp281, maternal-to-zygotic transition, pure zygotic, quantitative proteomics, tandem mass tag, znf281b
- Datasets
- GEO:GSE261646
- MeSH Terms
-
- Zygote/metabolism
- Gene Expression Regulation, Developmental*
- Animals
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- CRISPR-Cas Systems/genetics
- Embryo, Nonmammalian/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Embryonic Development*/genetics
- PubMed
- 39302832 Full text @ Cell Rep.
Citation
da Silva Pescador, G., Baia Amaral, D., Varberg, J.M., Zhang, Y., Hao, Y., Florens, L., Bazzini, A.A. (2024) Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis. Cell Reports. 43:114769114769.
Abstract
The maternal-to-zygotic transition is crucial in embryonic development, marked by the degradation of maternally provided mRNAs and initiation of zygotic gene expression. However, the changes occurring at the protein level during this transition remain unclear. Here, we conducted protein profiling throughout zebrafish embryogenesis using quantitative mass spectrometry, integrating transcriptomics and translatomics datasets. Our data show that, unlike RNA changes, protein changes are less dynamic. Further, increases in protein levels correlate with mRNA translation, whereas declines in protein levels do not, suggesting active protein degradation processes. Interestingly, proteins from pure zygotic genes are present at fertilization, challenging existing mRNA-based gene classifications. As a proof of concept, we utilized CRISPR-Cas13d to target znf281b mRNA, a gene whose protein significantly accumulates within the first 2 h post-fertilization, demonstrating its crucial role in development. Consequently, our protein profiling, coupled with CRISPR-Cas13d, offers a complementary approach to unraveling maternal factor function during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping