PUBLICATION
Differential vegfc expression dictates lymphatic response during zebrafish heart development and regeneration
- Authors
- Duca, S., Xia, Y., Elmagid, L.A., Bakis, I., Qiu, M., Cao, Y., Guo, Y., Eichenbaum, J.V., McCain, M.L., Kang, J., Harrison, M.R.M., Cao, J.
- ID
- ZDB-PUB-241114-50
- Date
- 2024
- Source
- Development (Cambridge, England) 151(22): (Journal)
- Registered Authors
- Cao, Jingli, Harrison, Michael, Kang, Junsu
- Keywords
- Coronary artery, Heart regeneration, Lymphatic vessel, Thrombocytes, Vegfc, Zebrafish
- MeSH Terms
-
- Coronary Vessels/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Myocytes, Cardiac/metabolism
- Heart*/physiology
- Regeneration*/physiology
- Vascular Endothelial Growth Factor C*/genetics
- Vascular Endothelial Growth Factor C*/metabolism
- Blood Platelets/metabolism
- Animals
- Lymphatic Vessels/metabolism
- Cell Proliferation
- Animals, Genetically Modified
- Gene Expression Regulation, Developmental
- Lymphangiogenesis*/genetics
- Zebrafish*
- PubMed
- 39514676 Full text @ Development
Citation
Duca, S., Xia, Y., Elmagid, L.A., Bakis, I., Qiu, M., Cao, Y., Guo, Y., Eichenbaum, J.V., McCain, M.L., Kang, J., Harrison, M.R.M., Cao, J. (2024) Differential vegfc expression dictates lymphatic response during zebrafish heart development and regeneration. Development (Cambridge, England). 151(22):.
Abstract
Vascular endothelial growth factor C (Vegfc) is crucial for lymphatic and blood vessel development, yet its cellular sources and specific functions in heart development remain unclear. To address this, we created a vegfc reporter and an inducible overexpression line in zebrafish. We found vegfc expression in large coronary arteries, circulating thrombocytes, cardiac adipocytes, and outflow tract smooth muscle cells. Notably, while coronary lymphangiogenesis aligns with Vegfc-expressing arteries in juveniles, it occurs only after coronary artery formation. Vegfc overexpression induced ectopic lymphatics on the ventricular surface prior to arterial formation, indicating that Vegfc abundance, rather than arterial presence, drives lymphatic development. However, this overexpression did not affect coronary artery coverage, suggesting a specific role for Vegfc in lymphatic, rather than arterial, development. Thrombocytes emerged as the initial Vegfc source during inflammation following heart injuries, transitioning to endocardial and myocardial expression during regeneration. Lower Vegfc levels in the amputation model corresponded with a lack of lymphatic expansion. Importantly, Vegfc overexpression enhanced lymphatic expansion and promoted scar resolution without affecting cardiomyocyte proliferation, highlighting its role in regulating lymphangiogenesis and promoting heart regeneration.
Errata / Notes
This article is corrected by ZDB-PUB-250411-2.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping