PUBLICATION
Pathogenic proteotoxicity of cryptic splicing is alleviated by ubiquitination and ER-phagy
- Authors
- Prieto-Garcia, C., Matkovic, V., Mosler, T., Li, C., Liang, J., Oo, J.A., Haidle, F., Mačinković, I., Cabrera-Orefice, A., Berkane, R., Giuliani, G., Xu, F., Jacomin, A.C., Tomaskovic, I., Basoglu, M., Hoffmann, M.E., Rathore, R., Cetin, R., Boutguetait, D., Bozkurt, S., Hernández Cañás, M.C., Keller, M., Busam, J., Shah, V.J., Wittig, I., Kaulich, M., Beli, P., Galej, W.P., Ebersberger, I., Wang, L., Münch, C., Stolz, A., Brandes, R.P., Tse, W.K.F., Eimer, S., Stainier, D.Y.R., Legewie, S., Zarnack, K., Müller-McNicoll, M., Dikic, I.
- ID
- ZDB-PUB-241115-5
- Date
- 2024
- Source
- Science (New York, N.Y.) 386: 768776768-776 (Journal)
- Registered Authors
- Stainier, Didier
- Keywords
- none
- Datasets
- GEO:GSE272836
- MeSH Terms
-
- Ubiquitin/metabolism
- Proteasome Endopeptidase Complex/metabolism
- HEK293 Cells
- Mice
- Ubiquitination*
- Autophagy*
- Zebrafish/genetics
- Humans
- Endoplasmic Reticulum Stress*
- Endoplasmic Reticulum*/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- RNA Splicing*
- Retinitis Pigmentosa*/genetics
- Retinitis Pigmentosa*/metabolism
- Ubiquitin-Specific Proteases*/genetics
- Ubiquitin-Specific Proteases*/metabolism
- HeLa Cells
- Animals
- RNA Splice Sites
- Spliceosomes/metabolism
- Protein Folding
- PubMed
- 39541449 Full text @ Science
Citation
Prieto-Garcia, C., Matkovic, V., Mosler, T., Li, C., Liang, J., Oo, J.A., Haidle, F., Mačinković, I., Cabrera-Orefice, A., Berkane, R., Giuliani, G., Xu, F., Jacomin, A.C., Tomaskovic, I., Basoglu, M., Hoffmann, M.E., Rathore, R., Cetin, R., Boutguetait, D., Bozkurt, S., Hernández Cañás, M.C., Keller, M., Busam, J., Shah, V.J., Wittig, I., Kaulich, M., Beli, P., Galej, W.P., Ebersberger, I., Wang, L., Münch, C., Stolz, A., Brandes, R.P., Tse, W.K.F., Eimer, S., Stainier, D.Y.R., Legewie, S., Zarnack, K., Müller-McNicoll, M., Dikic, I. (2024) Pathogenic proteotoxicity of cryptic splicing is alleviated by ubiquitination and ER-phagy. Science (New York, N.Y.). 386:768776768-776.
Abstract
RNA splicing enables the functional adaptation of cells to changing contexts. Impaired splicing has been associated with diseases, including retinitis pigmentosa, but the underlying molecular mechanisms and cellular responses remain poorly understood. In this work, we report that deficiency of ubiquitin-specific protease 39 (USP39) in human cell lines, zebrafish larvae, and mice led to impaired spliceosome assembly and a cytotoxic splicing profile characterized by the use of cryptic 5' splice sites. Disruptive cryptic variants evaded messenger RNA (mRNA) surveillance pathways and were translated into misfolded proteins, which caused proteotoxic aggregates, endoplasmic reticulum (ER) stress, and, ultimately, cell death. The detrimental consequence of splicing-induced proteotoxicity could be mitigated by up-regulating the ubiquitin-proteasome system and selective autophagy. Our findings provide insight into the molecular pathogenesis of spliceosome-associated diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping