PUBLICATION
Reelin/Disabled-1 Signalling Contributes to Functional Recovery on Zebrafish After Spinal Cord Injury
- Authors
- Li, R., Sahu, S.
- ID
- ZDB-PUB-250813-14
- Date
- 2025
- Source
- International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 85: e70045e70045 (Journal)
- Registered Authors
- Keywords
- Dab‐1, Reelin, locomotor behaviour, spinal cord injury, zebrafish
- MeSH Terms
-
- Reelin Protein
- Signal Transduction*/genetics
- Signal Transduction*/physiology
- Larva
- Animals
- Nerve Tissue Proteins*/genetics
- Nerve Tissue Proteins*/metabolism
- Zebrafish
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Axons
- Cell Adhesion Molecules, Neuronal*/genetics
- Cell Adhesion Molecules, Neuronal*/metabolism
- Extracellular Matrix Proteins*/genetics
- Extracellular Matrix Proteins*/metabolism
- Serine Endopeptidases*/genetics
- Serine Endopeptidases*/metabolism
- Recovery of Function*/genetics
- Recovery of Function*/physiology
- Disease Models, Animal
- Animals, Genetically Modified
- Spinal Cord Injuries*/metabolism
- Spinal Cord Injuries*/pathology
- Spinal Cord Injuries*/physiopathology
- PubMed
- 40798876 Full text @ Int. J. Dev. Neurosci.
Citation
Li, R., Sahu, S. (2025) Reelin/Disabled-1 Signalling Contributes to Functional Recovery on Zebrafish After Spinal Cord Injury. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 85:e70045e70045.
Abstract
Reelin, an extracellular matrix glycoprotein, plays important roles in neural development. Mutation-induced loss of its functions in mammals leads to severe disorders associated with impaired motor coordination, tremors and ataxia. Little is known about Reelin's role in functional recovery after central nervous system injury. We determined the effect of knock-down of Reelin and its downstream signal-transducing molecule Disabled-1 (Dab-1) on functional recovery after spinal cord injury in larval zebrafish. Larvae deficient in Reelin and Dab-1 were generated by application of two non-overlapping antisense morpholinos for each molecule. Individual knock-down of Reelin and Dab-1 expression impaired locomotor recovery after injury, inhibited remyelination of regrown axons and reduced numbers of motor neurons caudal to the lesion site. These results indicate that the Reelin/Dab-1 signalling pathway is involved in axon regeneration after injury of a paradigmatic vertebrate in the central nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping