PUBLICATION

Systematic Disruption of Zebrafish Fibrillin Genes Identifies a Translational Zebrafish Model for Marfan Syndrome

Authors
De Rycke, K., Horvat, M., Caboor, L., Vermassen, P., De Smet, G., Lobbestael, S., Santana Silva, M., Steyaert, W., Van Impe, M., Segers, P., De Backer, J., Sips, P.
ID
ZDB-PUB-260424-7
Date
2026
Source
JACC. Basic to translational science   11: 101543 (Journal)
Registered Authors
Sips, Patrick
Keywords
CRISPR/Cas9, animal disease models, aortic disease, bulbus arteriosus, extracellular matrix, fibrillinopathies
Datasets
GEO:GSE300393
MeSH Terms
none
PubMed
42025244 Full text @ JACC Basic Transl Sci
Abstract
Fibrillin defects lead to severe cardiovascular complications in Marfan syndrome (MFS), including aortic dilation, dissection, and rupture. To model MFS, zebrafish mutants lacking various fibrillin genes were generated. Among these mutant lines, only fibrillin-3-deficient zebrafish exhibited cardiovascular phenotypes mimicking human disease. Multimodal imaging revealed early cardiac defects, bulbus arteriosus dilation, and valve abnormalities. Transcriptomic analysis identified altered regulation of pathways related to extracellular matrix homeostasis and immune system activation. This zebrafish model, recapitulating key cardiovascular features of MFS, provides a valuable platform to investigate disease mechanisms and identify novel treatment strategies.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping