PUBLICATION
Zebrafish pax[zf-a]: a paired box-containing gene expressed in the neural tube
- Authors
- Krauss, S., Johansen, T., Korzh, V., Moens, U., Ericson, J.U., and Fjose, A.
- ID
- ZDB-PUB-961014-635
- Date
- 1991
- Source
- The EMBO journal 10: 3609-3619 (Journal)
- Registered Authors
- Fjose, Anders, Johansen, Terje, Korzh, Vladimir, Krauss, Stefan
- Keywords
- none
- MeSH Terms
-
- Rhombencephalon/chemistry
- Rhombencephalon/embryology
- Transcription, Genetic
- Cloning, Molecular
- Molecular Sequence Data
- DNA
- RNA/genetics
- Spinal Cord/chemistry
- Spinal Cord/embryology
- Genes, Regulator*
- Nucleic Acid Hybridization
- Eye/chemistry
- Sequence Homology, Nucleic Acid
- Base Sequence
- Nervous System/chemistry
- Nervous System/embryology*
- Zebrafish/embryology
- Zebrafish/genetics
- Animals
- Gene Expression
- Amino Acid Sequence
- Blotting, Northern
- PubMed
- 1718739 Full text @ EMBO J.
Citation
Krauss, S., Johansen, T., Korzh, V., Moens, U., Ericson, J.U., and Fjose, A. (1991) Zebrafish pax[zf-a]: a paired box-containing gene expressed in the neural tube. The EMBO journal. 10:3609-3619.
Abstract
Murine and human sequences homologous to the paired box of the Drosophila segmentation gene paired have been reported previously. Here we describe a zebrafish (Brachydanio rerio) paired box-containing clone, pax[zf-a], which is clearly distinct from reported vertebrate Pax genes. The putative protein encoded by pax[zf-a] contains a paired box and a paired-type homeobox separated by a glycine-rich, acidic linker and a carboxy-terminal end which is remarkably rich in serine, threonine and proline residues. By in situ hybridization to embryonic tissue sections and whole mount embryos, pax[zf-a] transcripts were found within restricted regions of the central nervous system and the eye. In contrast to the murine Pax genes recently characterized, pax[zf-a] is not expressed in the segmented mesoderm. At the 17 h stage, pax[zf-a] expression is detected in a defined area of the diencephalon which circumscribes the presumptive thalamus. This suggests an involvement of pax[zf-a] in pattern formation in the rostral brain. The pax[zf-a] gene is also expressed throughout the hindbrain and spinal cord. This hybridization signal is restricted to a longitudinal column which includes the basal plate. Later in development, at 36 h post-fertilization, pax[zf-a] transcripts are no longer restricted to a specific region of the diencephalon, but are distributed over the entire developing brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping