PUBLICATION
Development of branchiomotor neurons in zebrafish
- Authors
- Chandrasekhar, A., Moens, C.B., Warren, J.T., Kimmel, C.B., and Kuwada, J.Y.
- ID
- ZDB-PUB-970812-16
- Date
- 1997
- Source
- Development (Cambridge, England) 124(13): 2633-2644 (Journal)
- Registered Authors
- Chandrasekhar, Anand, Kimmel, Charles B., Kuwada, John, Moens, Cecilia, Warren, James T., Jr.
- Keywords
- zebrafish; hindbrain; branchiomotor neuron; axonogenesis; valentino; cyclops
- MeSH Terms
-
- Neural Tube Defects/genetics
- Body Patterning
- DNA-Binding Proteins/biosynthesis
- DNA-Binding Proteins/genetics
- Zebrafish/embryology
- Maf Transcription Factors
- Rhombencephalon/cytology
- Rhombencephalon/embryology*
- Contactin 2
- Animals
- Neural Pathways/embryology*
- Branchial Region/embryology*
- Branchial Region/innervation*
- Gene Expression
- Motor Neurons*
- Embryonic Induction
- Transcription Factors/biosynthesis
- Transcription Factors/genetics
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/genetics
- Mutation
- Zebrafish Proteins
- Avian Proteins*
- MafB Transcription Factor
- Cell Adhesion Molecules, Neuronal/biosynthesis
- Cell Adhesion Molecules, Neuronal/genetics
- Cell Movement
- Oncogene Proteins*
- Homeodomain Proteins/biosynthesis
- Homeodomain Proteins/genetics
- PubMed
- 9217005 Full text @ Development
Citation
Chandrasekhar, A., Moens, C.B., Warren, J.T., Kimmel, C.B., and Kuwada, J.Y. (1997) Development of branchiomotor neurons in zebrafish. Development (Cambridge, England). 124(13):2633-2644.
Abstract
The mechanisms underlying neuronal specification and axonogenesis in the vertebrate hindbrain are poorly understood. To address these questions, we have employed anatomical methods and mutational analysis to characterize the branchiomotor neurons in the zebrafish embryo. The zebrafish branchiomotor system is similar to those in the chick and mouse, except for the location of the nVII and nIX branchiomotor neurons. Developmental analyses of genes expressed by branchiomotor neurons suggest that the different location of the nVII neurons in the zebrafish may result from cell migration. To gain insight into the mechanisms underlying the organization and axonogenesis of these neurons, we examined the development of the branchiomotor pathways in neuronal mutants. The valentino b337 mutation blocks the formation of rhombomeres 5 and 6, and severely affects the development of the nVII and nIX motor nuclei. The cyclops b16 mutation deletes ventral midline cells in the neural tube, and leads to a severe disruption of most branchiomotor nuclei and axon pathways. These results demonstrate that rhombomere- specific cues and ventral midline cells play important roles in the development of the branchiomotor pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping