PUBLICATION
Positive and Negative Regulation of Muscle Cell Identity by Members of the hedgehog and TGF-β Gene Families
- Authors
- Du, S.J., S.H. Devoto, M. Westerfield, and R.T. Moon
- ID
- ZDB-PUB-971016-36
- Date
- 1997
- Source
- The Journal of cell biology 139(1): 145-156 (Journal)
- Registered Authors
- Devoto, Stephen Henri, Du, Shao Jun (Jim), Moon, Randall T., Westerfield, Monte
- Keywords
- none
- MeSH Terms
-
- Multigene Family
- Transforming Growth Factor beta/genetics*
- Transforming Growth Factor beta/physiology
- Up-Regulation/genetics*
- Signal Transduction
- Drosophila
- Muscle Fibers, Skeletal/cytology
- Muscle Fibers, Skeletal/enzymology
- Muscle Fibers, Skeletal/physiology*
- Zebrafish
- Cell Differentiation
- Embryo, Nonmammalian
- Notochord/metabolism
- Transforming Growth Factors/biosynthesis
- Transforming Growth Factors/physiology
- Muscle Fibers, Slow-Twitch/cytology
- Muscle Fibers, Slow-Twitch/physiology
- Animals
- Hedgehog Proteins
- Down-Regulation/genetics*
- Drosophila Proteins*
- Gene Expression Regulation, Developmental*
- Zebrafish Proteins*
- Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
- Cyclic AMP-Dependent Protein Kinases/physiology
- Insect Proteins/biosynthesis
- Insect Proteins/genetics*
- Insect Proteins/physiology
- PubMed
- 9314535 Full text @ J. Cell Biol.
Citation
Du, S.J., S.H. Devoto, M. Westerfield, and R.T. Moon (1997) Positive and Negative Regulation of Muscle Cell Identity by Members of the hedgehog and TGF-β Gene Families. The Journal of cell biology. 139(1):145-156.
Abstract
We have examined whether the development of embryonic muscle fiber type is regulated by competing influences between Hedgehog and TGF-beta signals, as previously shown for development of neuronal cell identity in the neural tube. We found that ectopic expression of Hedgehogs or inhibition of protein kinase A in zebrafish embryos induces slow muscle precursors throughout the somite but muscle pioneer cells only in the middle of the somite. Ectopic expression in the notochord of Dorsalin-1, a member of the TGF-beta superfamily, inhibits the formation of muscle pioneer cells, demonstrating that TGF-beta signals can antagonize the induction of muscle pioneer cells by Hedgehog. We propose that a Hedgehog signal first induces the formation of slow muscle precursor cells, and subsequent Hedgehog and TGF-beta signals exert competing positive and negative influences on the development of muscle pioneer cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping