PUBLICATION
Zebrafish serotonin N-acetyltransferase-2: marker for development of pineal photoreceptors and circadian clock function
- Authors
- Gothilf, Y., Coon, S.L., Toyama, R., Chitnis, A., Namboodiri, M.A.A., and Klein, D.C.
- ID
- ZDB-PUB-991014-3
- Date
- 1999
- Source
- Endocrinology 140(10): 4895-4903 (Journal)
- Registered Authors
- Chitnis, Ajay, Gothilf, Yoav, Klein, David C., Toyama, Reiko
- Keywords
- none
- MeSH Terms
-
- DNA, Complementary/genetics
- Molecular Sequence Data
- Retina/metabolism
- Animals
- Biomarkers
- Mutation/physiology
- Embryo, Nonmammalian/metabolism
- Circadian Rhythm/physiology*
- Photoreceptor Cells, Vertebrate/physiology*
- Isoenzymes/metabolism*
- RNA, Messenger/metabolism
- Pineal Gland/embryology*
- Pineal Gland/metabolism
- Arylamine N-Acetyltransferase/genetics
- Arylamine N-Acetyltransferase/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 10499549 Full text @ Endocrinology
Citation
Gothilf, Y., Coon, S.L., Toyama, R., Chitnis, A., Namboodiri, M.A.A., and Klein, D.C. (1999) Zebrafish serotonin N-acetyltransferase-2: marker for development of pineal photoreceptors and circadian clock function. Endocrinology. 140(10):4895-4903.
Abstract
Serotonin N-acetyltransferase (AANAT), the penultimate enzyme in melatonin synthesis, is typically found only at significant levels in the pineal gland and retina. Large changes in the activity of this enzyme drive the circadian rhythm in circulating melatonin seen in all vertebrates. In this study, we examined the utility of using AANAT messenger RNA (mRNA) as a marker to monitor the very early development of pineal photoreceptors and circadian clock function in zebrafish. Zebrafish AANAT-2 (zfAANAT-2) cDNA was isolated and used for in situ hybridization. In the adult, zfAANAT-2 mRNA is expressed exclusively in pineal cells and retinal
photoreceptors. Developmental analysis, using whole mount in situ hybridization, indicated that pineal zfAANAT-2 mRNA expression is first detected at 22 h post fertilization. Retinal zfAANAT-2 mRNA was first detected on day 3 post fertilization and appears to be associated with development of the retinal photoreceptors. Time-of-day analysis of 2- to 5-day-old zebrafish larvae indicated that zfAANAT-2 mRNA abundance exhibits a dramatic 24-h rhythm in a 14-h light, 10-h dark cycle, with high levels at night. This rhythm persists in constant darkness, indicating that the zfAANAT-2 mRNA rhythm is driven by a circadian clock at this stage. The techniques described in this report were also used to determine that zfAANAT-2 expression is altered in two well characterized genetic mutants, mindbomb and floating head. The observations described here suggest that zfAANAT-2 mRNA may be a useful marker to study development of the pineal gland and of circadian clock mechanisms in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping