Transgenic Construct

Tg(BMPRE-AAV.Mlp:d2EGFP)

ID

ZDB-TGCONSTRCT-110310-1

Previous Names

BRE-AAVmlp:d2GFP (1)
Tg(BmpRE-AAVmlp:d2EGFP)
Tg(Mmu.Id1-AAVmlp:d2EGFP) (1)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

d2EGFP (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

BMPRE (1)

Note

This construct utilizes the 84bp BMP Response Element (BRE) derived from mouse Id1 promoter, containing multiple short Smad-binding sites, and the Adeno-associated virus (AAV) major late promoter (mlp) driving expression of destabilized EGFP with a C-terminal PEST domain from mouse ornithine decarboxylase.

Plasmid Map

None

Genomic Features

That Utilize Tg(BMPRE-AAV.Mlp:d2EGFP) Construct

Genomic Feature	Affected Genomic Regions
mw30Tg

Transgenics

That Utilize Tg(BMPRE-AAV.Mlp:d2EGFP) Construct

Fish	Phenotype	Gene Expression
hu7478Tg/hu7478Tg; mw30Tg/mw30Tg	Fig. 1 from Peskin et al., 2023	2 figures from Peskin et al., 2023
hu7478Tg/hu7478Tg; mw30Tg/mw30Tg + CRISPR2-bmp3 + CRISPR3-bmp3 + CRISPR4-bmp3 + CRISPR5-bmp3 + CRISPR6-bmp3 + CRISPR7-bmp3		Fig. 2 from Peskin et al., 2023
hu7478Tg/hu7478Tg; mw30Tg/mw30Tg + CRISPR2-nog3 + CRISPR3-nog3 + CRISPR4-nog3 + CRISPR5-nog3 + CRISPR6-nog3 + CRISPR7-nog3	Fig. 4 from Peskin et al., 2023	Fig. 4 from Peskin et al., 2023
mw30Tg		2 figures from 2 publications
mw30Tg + MO1-hs3st1l1		Fig. 4 from Samson et al., 2013
mw30Tg + MO2-hs3st1l1		Fig. 4 from Samson et al., 2013
mw30Tg + MO6-sox2		Fig. 6 from Pavlou et al., 2014
mw30Tg/+ (AB)
mw30Tg/mw30Tg; vu234Tg/vu234Tg	Fig 6 from Swartz et al., 2021
mw30Tg; s903Tg		Fig. 1 from Lovely et al., 2016
mw30Tg; s957Tg		Figure 7 from Gálvez-Santisteban et al., 2019
mw30Tg; vu234Tg		Fig. 1 from Lovely et al., 2016

Sequence Information

Other Construct Pages

None

Citations