CRISPR

CRISPR1-kcnb1

ID

ZDB-CRISPR-170106-1

Name

CRISPR1-kcnb1

Previous Names

None

Target

kcnb1 (1)

Sequence

5' - GGAGCTGGACTACTGGGGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sq301	kcnb1

Expression

Gene expression in Wild Types + CRISPR1-kcnb1

No data available

Phenotype

Phenotype resulting from CRISPR1-kcnb1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-kcnb1

Phenotype	Fish	Conditions	Figures
swimming decreased linear velocity, abnormal	kcnb1^sq301/sq301	lighting conditions	Fig. 3 from Robichon et al., 2025
head glutamic acid normal amount, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 5 from Robichon et al., 2025
whole organism kcnb1 expression decreased amount, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 2 from Robichon et al., 2025
swimming decreased linear velocity, abnormal	kcnb1^sq301/sq301	acoustic radiation	Fig. 3 from Robichon et al., 2025
tectal ventricle decreased size, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 7 from Shen et al., 2016
swimming behavior disrupted, abnormal	kcnb1^sq301/sq301	lighting conditions	Fig. 3 from Robichon et al., 2025
response to auditory stimulus decreased magnitude, abnormal	kcnb1^sq301/sq301	acoustic radiation	Fig. 3 from Robichon et al., 2025
neuronal action potential increased frequency, exacerbated	kcnb1^sq301/sq301	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
neuronal action potential increased frequency, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 5 from Robichon et al., 2025
swimming increased linear velocity, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 3 , Fig. 4 from Robichon et al., 2025
epiboly delayed, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. S8 from Shen et al., 2016
brain ventricular system decreased size, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 7 from Shen et al., 2016
fourth ventricle decreased size, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 7 from Shen et al., 2016
whole organism bdnf expression increased amount, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 4 from Robichon et al., 2025
swimming increased frequency, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 3 from Robichon et al., 2025
head glutamic acid normal amount, abnormal	kcnb1^sq301/sq301	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
head gamma-aminobutyric acid increased amount, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 5 from Robichon et al., 2025
swimming behavior disrupted, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. 3 from Robichon et al., 2025
gastrulation disrupted, abnormal	kcnb1^sq301/sq301	standard conditions	Fig. S8 from Shen et al., 2016
head gamma-aminobutyric acid increased amount, abnormal	kcnb1^sq301/sq301	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
otolith increased amount, abnormal	kcnb1^sq301/sq301 (AB)	standard conditions	Fig. S1 from Jędrychowska et al., 2024
otic vesicle increased size, abnormal	kcnb1^sq301/sq301 (AB)	standard conditions	Fig. S1 from Jędrychowska et al., 2024
head glutamic acid normal amount, abnormal	kcnb1^sq301/+	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
whole organism kcnb1 expression decreased amount, abnormal	kcnb1^sq301/+	standard conditions	Fig. 2 from Robichon et al., 2025
neuronal action potential increased duration, abnormal	kcnb1^sq301/+	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
swimming increased linear velocity, abnormal	kcnb1^sq301/+	standard conditions	Fig. 3 , Fig. 4 from Robichon et al., 2025
neuronal action potential increased frequency, abnormal	kcnb1^sq301/+	chemical treatment by environment: pentetrazol	Fig. 5 from Robichon et al., 2025
swimming increased frequency, abnormal	kcnb1^sq301/+	standard conditions	Fig. 3 from Robichon et al., 2025
swimming behavior disrupted, abnormal	kcnb1^sq301/+	standard conditions	Fig. 3 from Robichon et al., 2025

Citations