CRISPR

CRISPR1-bdnf

ID

ZDB-CRISPR-220929-1

Name

CRISPR1-bdnf

Previous Names

None

Target

bdnf (1)

Sequence

5' - GGCGGCGCCCAGGTAGCCCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
fds1	bdnf

Expression

Gene expression in Wild Types + CRISPR1-bdnf

No data available

Phenotype

Phenotype resulting from CRISPR1-bdnf

No data available

Phenotype of all Fish created by or utilizing CRISPR1-bdnf

Phenotype	Fish	Conditions	Figures
whole organism swimming behavior process quality, ameliorated	bdnf^fds1/fds1 (AB)	chemical treatment by environment: 7,8-dihydroxyflavone	Fig. 3 from D'Agostino et al., 2022
whole organism circadian rhythm decreased process quality, abnormal	bdnf^fds1/fds1 (AB)	constant dark	Fig. 6 , Fig. 7 from D'Agostino et al., 2022
whole organism circadian behavior process quality, ameliorated	bdnf^fds1/fds1 (AB)	constant dark, chemical treatment by environment: 7,8-dihydroxyflavone	Fig. 7 from D'Agostino et al., 2022
whole organism mtnr1aa expression decreased amount, abnormal	bdnf^fds1/fds1 (AB)	constant dark	Fig. 5 from D'Agostino et al., 2022
whole organism swimming behavior decreased process quality, abnormal	bdnf^fds1/fds1 (AB)	standard conditions	Fig. 2 , Fig. 3 from D'Agostino et al., 2022
whole organism bmal1a expression decreased amount, abnormal	bdnf^fds1/fds1 (AB)	control	Fig. 4 from D'Agostino et al., 2022
whole organism locomotor rhythm decreased process quality, abnormal	bdnf^fds1/fds1 (AB)	constant dark	Fig. 6 , Fig. 7 from D'Agostino et al., 2022
whole organism aanat2 expression decreased amount, abnormal	bdnf^fds1/fds1 (AB)	constant dark	Fig. 5 from D'Agostino et al., 2022
whole organism Ab5-bdnf labeling decreased amount, abnormal	bdnf^fds1/fds1 (AB)	standard conditions	Fig. 1 from D'Agostino et al., 2022
whole organism locomotor rhythm process quality, ameliorated	bdnf^fds1/fds1 (AB)	constant dark, chemical treatment by environment: 7,8-dihydroxyflavone	Fig. 7 from D'Agostino et al., 2022
whole organism Ab5-bdnf labeling decreased amount, abnormal	bdnf^fds1/+ (AB)	standard conditions	Fig. 1 from D'Agostino et al., 2022

Citations