CRISPR

CRISPR1-cln8

ID

ZDB-CRISPR-250108-1

Name

CRISPR1-cln8

Previous Names

None

Target

cln8 (1)

Sequence

5' - GGTTTGCAGGGGCGGTTGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
irc2	cln8

Expression

Gene expression in Wild Types + CRISPR1-cln8

No data available

Phenotype

Phenotype resulting from CRISPR1-cln8

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cln8

Phenotype	Fish	Conditions	Figures
locomotory exploration behavior increased process quality, abnormal	cln8^irc2/irc2 (AB)	standard conditions	Figure 2 from Licitra et al., 2025
social behavior decreased process quality, abnormal	cln8^irc2/irc2 (AB)	chemical treatment by diet: trehalose	Figure 3 from Licitra et al., 2025
cognition decreased process quality, abnormal	cln8^irc2/irc2 (AB)	chemical treatment by diet: trehalose	Figure 7 from Licitra et al., 2025
social behavior process quality, ameliorated	cln8^irc2/irc2 (AB)	chemical treatment by diet: trehalose	Figure 4 from Licitra et al., 2025
aggressive behavior increased process quality, abnormal	cln8^irc2/irc2 (AB)	standard conditions	Figure 5 , Figure 6 from Licitra et al., 2025
aggressive behavior process quality, ameliorated	cln8^irc2/irc2 (AB)	chemical treatment by diet: trehalose	Figure 5 , Figure 6 from Licitra et al., 2025
social behavior decreased process quality, abnormal	cln8^irc2/irc2 (AB)	standard conditions	Figure 3 , Figure 4 from Licitra et al., 2025
locomotory exploration behavior process quality, ameliorated	cln8^irc2/irc2 (AB)	chemical treatment by diet: trehalose	Figure 2 from Licitra et al., 2025
cognition decreased process quality, abnormal	cln8^irc2/irc2 (AB)	standard conditions	Figure 7 from Licitra et al., 2025
locomotion increased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 2 , Fig. 7 from Marchese et al., 2024
swimming behavior decreased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 7 from Marchese et al., 2024
whole organism map1lc3b expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 from Marchese et al., 2024
locomotion process quality, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 7 from Marchese et al., 2024
trunk lysosomal lumen pH elevation increased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 3 from Marchese et al., 2024
whole organism hif1ab expression decreased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 , Fig. 8 from Marchese et al., 2024
trunk Ab1-atp5 labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 3 from Marchese et al., 2024
whole organism ab2-map1lc3 labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 , Fig. 8 from Marchese et al., 2024
whole organism Ab2-ctsd labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 8 from Marchese et al., 2024
swimming behavior decreased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 2 , Fig. 7 from Marchese et al., 2024
swimming behavior decreased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 7 from Marchese et al., 2024
whole organism tnfa expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 5 from Marchese et al., 2024
yolk increased area, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 1 from Marchese et al., 2024
eye decreased size, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 1 from Marchese et al., 2024
whole organism Ab4-rab7 labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 8 from Marchese et al., 2024
whole organism becn1 expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 from Marchese et al., 2024
whole organism il1b expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 5 from Marchese et al., 2024
whole organism ab2-map1lc3 labeling amount, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 8 from Marchese et al., 2024
whole organism cln8 expression decreased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 1 from Marchese et al., 2024
whole organism mtor expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 from Marchese et al., 2024
whole organism hif1ab expression amount, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 8 from Marchese et al., 2024
whole organism ab2-map1lc3 labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 8 from Marchese et al., 2024
head lipid increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 3 from Marchese et al., 2024
whole organism Ab4-rab7 labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 , Fig. 8 from Marchese et al., 2024
whole organism atg5 expression increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 from Marchese et al., 2024
whole organism increased length, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 1 from Marchese et al., 2024
whole organism hif1ab expression amount, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 8 from Marchese et al., 2024
brain lysosomal lumen pH elevation increased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 3 from Marchese et al., 2024
whole organism Ab2-ctsd labeling amount, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 8 from Marchese et al., 2024
brain apoptotic process increased process quality, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 3 from Marchese et al., 2024
whole organism Ab2-ctsd labeling increased amount, abnormal	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 6 , Fig. 8 from Marchese et al., 2024
whole organism Ab4-rab7 labeling amount, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 8 from Marchese et al., 2024
locomotion process quality, ameliorated	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 7 from Marchese et al., 2024
brain action potential initiation increased duration, abnormal	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 4 , Fig. 7 from Marchese et al., 2024
brain action potential initiation process quality, ameliorated	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 7 from Marchese et al., 2024
brain action potential initiation process quality, ameliorated	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 7 from Marchese et al., 2024
brain action potential initiation increased duration, abnormal	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: trehalose	Fig. 7 from Marchese et al., 2024
brain action potential initiation increased process quality, abnormal	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	standard conditions	Fig. 4 , Fig. 7 from Marchese et al., 2024
brain action potential initiation duration, ameliorated	mitfa^w2/w2; cln8^irc2/irc2; icm05Tg (AB)	chemical treatment by environment: 1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide	Fig. 7 from Marchese et al., 2024

Citations