CRISPR

CRISPR7-hspd1

ID

ZDB-CRISPR-250224-3

Name

CRISPR7-hspd1

Previous Names

None

Target

hspd1 (1)

Sequence

5' - AGTTTGGAGCAGATGCCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf4208	hspd1

Expression

Gene expression in Wild Types + CRISPR7-hspd1

No data available

Phenotype

Phenotype resulting from CRISPR7-hspd1

No data available

Phenotype of all Fish created by or utilizing CRISPR7-hspd1

Phenotype	Fish	Conditions	Figures
whole organism phenylalanine increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
swim bladder uninflated, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 4 from Cömert et al., 2024
whole organism serine increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
whole organism methionine increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
whole organism asparagine increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
whole organism lysine increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
eye decreased area, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 4 from Cömert et al., 2024
whole organism decreased length, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 4 from Cömert et al., 2024
whole organism hspd1 expression decreased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 4 from Cömert et al., 2024
whole organism glutamic acid increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
liver lipid increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 6 from Cömert et al., 2024
whole organism aspartate increased amount, abnormal	hspd1^{zf4208/zf4208} (AB)	standard conditions	Fig. 5 from Cömert et al., 2024
post-vent region oligodendrocyte decreased amount, abnormal	hspd1^{zf4208/zf4208}; zf2139Tg (AB)	standard conditions	Fig. 6 from Cömert et al., 2024

Citations