Fig. S1

Expression of {delta}113p53 is selectively up-regulated in the def^hi429 mutant. (A) RNA gel blot hybridization showed that p53 expression in the wild type peaked at 1 dpf and then gradually reduced to a lower level at 5 dpf (lanes 1 to 5). On the other hand, at 5 dpf, a short p53 isoform ({delta}113p53) (red arrow) rather than the wild type p53 (black arrow) expressed at a very high level in the def^hi429 mutant (mu) (lane 6). (B) In situ hybridization using P1 probe as in Fig. 5D showed elevated expression of {delta}113p53 in the def^hi429 mutant phenocopies the expression pattern displayed by def (Fig. 4C) at 2 dpf. wt: wild type; mu: mutant. (C) In situ hybridization using an {delta}113p53 specific probe (269 bp) derived from intron 4 (forward primer: 5′-CAGTGGAGGTTGAACATGTCT-3′; reverse primer: 5′-CCTCCTGGTCTTGTAATGTCAC-3′) containing the transcribed 143 bp in {delta}113p53 showed that the expression of {delta}113p53 is specifically enriched in the digestive organs in the def^hi429 mutant. wt: wild type; mu: mutant; in: intestine tube; lv: liver; p: pancreas; phy: pharyngeal.