Fig. 8

Eliminating Glomerular Filtration Results in Ectopic Tubule Convolution

All embryos were imaged at 84 hpf.

(A and B) Control condition, ET33-D10 transgenics (A) and ET11–9 transgenics (B).

(C and D) Cardiac tnnt2 morpholino injected embryos, ET33-D10 transgenic (C) and ET11–9 transgenic (D).

(E and F) Cardiac tnnt2 and ift88 (polaris) morpholino injected embryos, ET33d10 transgenic (E) and ET11–9 transgenic (F).

(G–M) Anti-alpha6F NaK ATPase stained embryos. (G) Control embryo. (H and I) Close-ups of the proximal and mid segments. (J) Cardiac tnnt2 morpholino injected embryo. (K and L) Close-ups of the proximal and the mid segments. (M) Cardiac tnnt2 and ift88(polaris) morpholino-injected embryo.

(N) ET11–9 GFP transgenic embryo injected with cardiac tnnt2 morpholino, stained with anti-alpha6 NaK ATPase (faint), and anti-GFP (bright).