FIGURE

Fig. 2

ID

ZDB-FIG-101123-65

Publication

Chung et al., 2010 - Suppression of Alk8-mediated Bmp signaling cell-autonomously induces pancreatic β-cells in zebrafish

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Fig. 2

Suppression of Bmp signaling is sufficient to induce ectopic dorsal bud-derived pancreatic β-cells cell-autonomously. (A) Schematic diagram of the cell transplantation protocol. Tg(ins:GFP) donors were injected with cas/sox32 and dn-alk8 RNA along with rhodamine dextran (red), and the cells were transplanted into Tg(ins:GFP) hosts. (B–E) Ventral confocal images of Tg(ins:GFP) (green), β-catenin (white), and rhodamine dextran (red) at 18 hpf (the notochord is outlined by yellow dashed lines). (B) In control embryos, Tg(ins:GFP)-expressing cells are located close to the notochord. (C and D) Ectopic Tg(ins:GFP)-expressing cells (arrows) were found in lateral and anterior endodermal regions where dn-Alk8-expressing cells had incorporated, and all these ectopic cells were donor derived. (E) Gremlin1a overexpression in the endoderm also resulted in the ectopic formation of Tg(ins:GFP)-expressing cells (arrows). (F) When Hedgehog signaling was blocked with cyclopamine, Tg(ins:GFP)-expressing cells were almost absent in hosts containing control donor cells. (G) dn-Alk8 expression still induced the formation of Tg(ins:GFP)-expressing cells (arrows) even after cyclopamine treatment, and it did so cell-autonomously.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA