FIGURE

Fig. S5

ID

ZDB-FIG-121029-8

Publication

Ni et al., 2012 - Conditional control of gene function by an invertible gene trap in zebrafish

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Fig. S5

Additional evidence for hepatocyte-specific inactivation of supv3l1 in supv3l1^GtN/GtN, hepatocyte-Cre fish. (A) No mCherry was detected in 18-d-old supv3l1^GtN/GtN fish, including in the liver. (B) mCherry was detected only in hepatocytes of 18-d-old supv3l1^GtN/GtN, hepatocyte-Cre fish, but not in the 2F11+ ductal cells or in intestinal cells. GL, gut lumen; L, liver; K, kidney head. (C) Relative levels of supv3l1 transcripts in the liver of 18-d-old supv3l1^GtN/GtN and supv3l1^GtN/GtN, hepatocyte-Cre fish were determined by RT-PCR. Error bar = SEM. (D) Relative levels of supv3l1^GtT allele and supv3l1^GtN allele in the liver of 30-dold LiPan+//hepatocyte-Cre+// supv3l1^GtNf/+ and LiPan/supv3l1^LKO fish were determined by qPCR. Error bar = SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA