FIGURE

Fig. 6

ID

ZDB-FIG-130503-33

Publication

Lin et al., 2013 - The Critical Role of Protein Arginine Methyltransferase prmt8 in Zebrafish Embryonic and Neural Development Is Non-Redundant with Its Paralogue prmt1

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Fig. 6

Characterization of perturbed brain phenotypes in the prmt8 morphants.

Zebrafish embryos (24 hpf) injected with 2 ng of prmt8 MO2, or 2 ng of MO2 together with full-length (FL) prmt8 cRNA, prmt8 N-terminus with full-length prmt1 (prmt8+1) cRNA, N-terminus-deleted (ND) prmt8 cRNA, catalytically inactive mutant (CI MT) prmt8 cRNA or G2A mutated prmt8 cRNA, were stained with otx2 (A; dorsal view with anterior to the top) or pax2.1 (B; lateral view with anterior to the left). Arrows indicate aberrant expression patterns at the mid-hindbrain boundaries in morphant embryos. Dash lines indicate the relative length of the dorsoventral axis of the developing brain. (C) Typical embryos from the Tg(huC::eGFP) fish, images were taken by Leica DM2500 epifluorescence microscope with a DFC490 CCD camera.

Expression Data

Genes:	otx2b pax2a
Fish:	WT WT + MO2-prmt8b
Knockdown Reagent:	MO2-prmt8b
Anatomical Terms:	midbrain midbrain hindbrain boundary
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-prmt8b
Knockdown Reagent:	MO2-prmt8b
Observed In:	brain dorsal-ventral axis brain neuron central nervous system neuron development midbrain spinal cord neuron
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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